Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7170 | 3' | -61.6 | NC_001900.1 | + | 45541 | 0.69 | 0.204658 |
Target: 5'- -cGCcGCgUCGAUgGCGuccuggauGUCACCGGCCa -3' miRNA: 3'- aaCGaCGgAGCUA-CGC--------CGGUGGCCGG- -5' |
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7170 | 3' | -61.6 | NC_001900.1 | + | 37766 | 0.68 | 0.238337 |
Target: 5'- -aGCUGCagagCGcguuccuGUGCGGCCuuuCCgGGCCa -3' miRNA: 3'- aaCGACGga--GC-------UACGCCGGu--GG-CCGG- -5' |
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7170 | 3' | -61.6 | NC_001900.1 | + | 39921 | 0.68 | 0.238946 |
Target: 5'- -gGCUGUCaUCGGa-CGGCCAccacCCGGCCc -3' miRNA: 3'- aaCGACGG-AGCUacGCCGGU----GGCCGG- -5' |
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7170 | 3' | -61.6 | NC_001900.1 | + | 41761 | 0.66 | 0.353772 |
Target: 5'- -gGUUGUagaGGUGCGGCCACUuGUCg -3' miRNA: 3'- aaCGACGgagCUACGCCGGUGGcCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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