miRNA: 3'- aaCGA-CGG----AGCUa-----------CGCCGGUgGCCGG- -5'
miRNA: 3'- aaCGa---CGg--AGCUACG--CCGGUGG-CCGG- -5'
miRNA: 3'- aaCGAC--------GGAGCUACG-------------CCGG---UGGCCGG- -5'
miRNA: 3'- aaCGACGgagCUACGCCGGUGGcCGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7170 | 3' | -61.6 | NC_001900.1 | + | 4336 | 0.66 | 0.343939 |
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Target: 5'- -aGCUcGCUcggaUCGAcaagguguggcaGCGGCCAgCGGCCu -3' miRNA: 3'- aaCGA-CGG----AGCUa-----------CGCCGGUgGCCGG- -5' |
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| 7170 | 3' | -61.6 | NC_001900.1 | + | 21621 | 0.66 | 0.345564 |
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Target: 5'- -cGCgucgGCacaUCGGUGCucGGCUACCcGGUCg -3' miRNA: 3'- aaCGa---CGg--AGCUACG--CCGGUGG-CCGG- -5' |
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| 7170 | 3' | -61.6 | NC_001900.1 | + | 35702 | 0.66 | 0.352945 |
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Target: 5'- -cGCUGgacgaugaCCUUGGUGCccuucuucacgucGGCCuccGCUGGCCg -3' miRNA: 3'- aaCGAC--------GGAGCUACG-------------CCGG---UGGCCGG- -5' |
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| 7170 | 3' | -61.6 | NC_001900.1 | + | 41761 | 0.66 | 0.353772 |
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Target: 5'- -gGUUGUagaGGUGCGGCCACUuGUCg -3' miRNA: 3'- aaCGACGgagCUACGCCGGUGGcCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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