Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7175 | 3' | -50.9 | NC_001900.1 | + | 31500 | 0.67 | 0.851912 |
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Target: 5'- aCACc-GAAGUCAGCG-CGGGCAUCAg -3' miRNA: 3'- gGUGccCUUUAGUCGUaGUCUGUGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 31349 | 0.68 | 0.824488 |
|
Target: 5'- aCCACGG----UCAGCA-CAGGCcCCAg -3' miRNA: 3'- -GGUGCCcuuuAGUCGUaGUCUGuGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 29152 | 0.68 | 0.795105 |
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Target: 5'- gUACGGGggGaUCAuguuGCGgaacCGGACACCGu -3' miRNA: 3'- gGUGCCCuuU-AGU----CGUa---GUCUGUGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 45492 | 0.7 | 0.675845 |
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Target: 5'- aCGCGGGGAA-CAGCGagacCGGauGCACCAg -3' miRNA: 3'- gGUGCCCUUUaGUCGUa---GUC--UGUGGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 31118 | 0.73 | 0.528737 |
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Target: 5'- aUCGCGGG-GAUCAGCGUCuGGaACGCUg -3' miRNA: 3'- -GGUGCCCuUUAGUCGUAG-UC-UGUGGu -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 5911 | 0.77 | 0.340996 |
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Target: 5'- uCCugGGGGAcAUCAGC-UCAGGCGgCAc -3' miRNA: 3'- -GGugCCCUU-UAGUCGuAGUCUGUgGU- -5' |
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| 7175 | 3' | -50.9 | NC_001900.1 | + | 27511 | 0.66 | 0.913585 |
|
Target: 5'- gUACGGug---CcGCGUCAGACACCu -3' miRNA: 3'- gGUGCCcuuuaGuCGUAGUCUGUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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