Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7214 | 3' | -52 | NC_001900.1 | + | 25754 | 0.66 | 0.879345 |
Target: 5'- gCCcAGCcggGGCCGAC-CGCCG-UGGUg -3' miRNA: 3'- -GGcUUGua-CUGGUUGaGCGGCuACCA- -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 41972 | 0.66 | 0.879345 |
Target: 5'- gCCGAAgccGuCCGACUCGC-GAUGGa -3' miRNA: 3'- -GGCUUguaCuGGUUGAGCGgCUACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 15942 | 0.66 | 0.878564 |
Target: 5'- aCCGggUgaccaagAUGGCCGaucACUCGCCaaccugGGUGGc -3' miRNA: 3'- -GGCuuG-------UACUGGU---UGAGCGG------CUACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 42745 | 0.66 | 0.871409 |
Target: 5'- gCCGuGGCcgGugCGGCUgCGCCGAccaGGUu -3' miRNA: 3'- -GGC-UUGuaCugGUUGA-GCGGCUa--CCA- -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 24810 | 0.66 | 0.871409 |
Target: 5'- cUCGGGCgGUGGCacGCUCaucgcuGCCGGUGGUg -3' miRNA: 3'- -GGCUUG-UACUGguUGAG------CGGCUACCA- -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 46110 | 0.66 | 0.846077 |
Target: 5'- gCCGcgaagguaGAUGUGACCuccaUCGCCGAgcUGGUc -3' miRNA: 3'- -GGC--------UUGUACUGGuug-AGCGGCU--ACCA- -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 21064 | 0.67 | 0.818635 |
Target: 5'- cCCGAacgGCGUGAUCuucguGCcCGCCGggGGc -3' miRNA: 3'- -GGCU---UGUACUGGu----UGaGCGGCuaCCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 24411 | 0.67 | 0.809065 |
Target: 5'- cCUGAGCGUGGCCGucacccaggaAC-CGCCGAagccgccuUGGc -3' miRNA: 3'- -GGCUUGUACUGGU----------UGaGCGGCU--------ACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 39418 | 0.67 | 0.7993 |
Target: 5'- gCGAGCA--GCCuugAACUC-CCGGUGGUg -3' miRNA: 3'- gGCUUGUacUGG---UUGAGcGGCUACCA- -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 39002 | 0.67 | 0.798314 |
Target: 5'- gUGAAguUGGCCAGCUCggcuGCCGAgaacugcUGGa -3' miRNA: 3'- gGCUUguACUGGUUGAG----CGGCU-------ACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 3925 | 0.68 | 0.789353 |
Target: 5'- gCGAGC-UGGCCGAgaCGCCGGUa-- -3' miRNA: 3'- gGCUUGuACUGGUUgaGCGGCUAcca -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 47264 | 0.68 | 0.779235 |
Target: 5'- cUCG-ACAUcAgCGAgUCGCCGAUGGUc -3' miRNA: 3'- -GGCuUGUAcUgGUUgAGCGGCUACCA- -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 11327 | 0.68 | 0.768958 |
Target: 5'- aCCG-ACAUGGCCggUUaCGCCGGUu-- -3' miRNA: 3'- -GGCuUGUACUGGuuGA-GCGGCUAcca -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 36442 | 0.68 | 0.747973 |
Target: 5'- gCCucACcgGGCCGcaGCUCGCCGAgcuucuucUGGa -3' miRNA: 3'- -GGcuUGuaCUGGU--UGAGCGGCU--------ACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 32982 | 0.69 | 0.693575 |
Target: 5'- gCCGAACuugucGAUCAGCUCGgCGA-GGg -3' miRNA: 3'- -GGCUUGua---CUGGUUGAGCgGCUaCCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 41441 | 0.69 | 0.693575 |
Target: 5'- aCCaGGCGU-ACCuGCUCGUCGGUGGg -3' miRNA: 3'- -GGcUUGUAcUGGuUGAGCGGCUACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 6906 | 0.69 | 0.690247 |
Target: 5'- aCGGGgAUGACCcguucagcggccugGAgUCGCUGAUGGa -3' miRNA: 3'- gGCUUgUACUGG--------------UUgAGCGGCUACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 12165 | 0.7 | 0.682462 |
Target: 5'- cCCGAGCAggcgaagGACUAcUUCGCCGA-GGc -3' miRNA: 3'- -GGCUUGUa------CUGGUuGAGCGGCUaCCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 6292 | 0.7 | 0.671298 |
Target: 5'- gCUGGACGccUGGCaCGACcuggUGCCGAUGGa -3' miRNA: 3'- -GGCUUGU--ACUG-GUUGa---GCGGCUACCa -5' |
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7214 | 3' | -52 | NC_001900.1 | + | 8693 | 0.7 | 0.671298 |
Target: 5'- aCGAGgAgaugcUGAcCCGGCUCGCCGAgcacgGGUu -3' miRNA: 3'- gGCUUgU-----ACU-GGUUGAGCGGCUa----CCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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