Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7254 | 3' | -53 | NC_001900.1 | + | 5479 | 0.72 | 0.466711 |
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Target: 5'- -------gUCGGCUCuACAAGGGCCGg -3' miRNA: 3'- ucauuauuGGCCGGGuUGUUCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 2532 | 0.68 | 0.687658 |
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Target: 5'- cGGUGGcAGcCCGGaCgCAGCcGGGGCCGu -3' miRNA: 3'- -UCAUUaUU-GGCC-GgGUUGuUCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 2416 | 0.67 | 0.709907 |
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Target: 5'- -----gAGCCGGCCCAACucacucuGcGGCUGc -3' miRNA: 3'- ucauuaUUGGCCGGGUUGuu-----C-CCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 1663 | 0.68 | 0.676443 |
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Target: 5'- -----aGGCgUGGUUCGGCAAGGGCCa -3' miRNA: 3'- ucauuaUUG-GCCGGGUUGUUCCCGGc -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 384 | 0.67 | 0.731832 |
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Target: 5'- --cAAUuccCCGGCCCuuguuGGGCCGg -3' miRNA: 3'- ucaUUAuu-GGCCGGGuuguuCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 302 | 1.1 | 0.001141 |
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Target: 5'- gAGUAAUAACCGGCCCAACAAGGGCCGg -3' miRNA: 3'- -UCAUUAUUGGCCGGGUUGUUCCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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