Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7348 | 3' | -51.4 | NC_001900.1 | + | 28010 | 0.65 | 0.906205 |
Target: 5'- aUCACGCCGuucgggccgguguAACCGggugCGGAG-CCGc- -3' miRNA: 3'- -AGUGCGGCu------------UUGGCa---GCUUCaGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15825 | 0.66 | 0.894932 |
Target: 5'- cCGCGCCGugGCUGgugcCGAAGUacgCCAGc -3' miRNA: 3'- aGUGCGGCuuUGGCa---GCUUCA---GGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 25252 | 0.66 | 0.871881 |
Target: 5'- -aGCGCCGAuAGCCGacCGGAGcCCGu- -3' miRNA: 3'- agUGCGGCU-UUGGCa-GCUUCaGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 21630 | 0.66 | 0.879835 |
Target: 5'- cCGCGUCGAAcgugacuCCGUCGAGGcgCUGAu -3' miRNA: 3'- aGUGCGGCUUu------GGCAGCUUCa-GGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 10969 | 0.66 | 0.894932 |
Target: 5'- -aACGCCGAGGCaagaCGuacGUCCAGGa -3' miRNA: 3'- agUGCGGCUUUGgca-GCuu-CAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 17470 | 0.66 | 0.901362 |
Target: 5'- ---gGCCGAAccgccagaagcucACCGagcgUGAGGUCCGGGa -3' miRNA: 3'- agugCGGCUU-------------UGGCa---GCUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 25391 | 0.66 | 0.902063 |
Target: 5'- -aACGCCG-AGCCGaCGAAGUggUCAGa -3' miRNA: 3'- agUGCGGCuUUGGCaGCUUCA--GGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 18950 | 0.66 | 0.871881 |
Target: 5'- gCGCuGUCGAucagGACCG-CGAGGaaUCCAAGa -3' miRNA: 3'- aGUG-CGGCU----UUGGCaGCUUC--AGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 45888 | 0.66 | 0.871881 |
Target: 5'- -gACGCUGAAG-CGUCG-GGUCCGc- -3' miRNA: 3'- agUGCGGCUUUgGCAGCuUCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 31517 | 0.66 | 0.887521 |
Target: 5'- uUCACGCCGuAGuuGUCGGcgAGaUCCu-- -3' miRNA: 3'- -AGUGCGGCuUUggCAGCU--UC-AGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15055 | 0.66 | 0.887521 |
Target: 5'- aUACGCCGGccgaacugaaGACCaUCGAccuGUCCGAc -3' miRNA: 3'- aGUGCGGCU----------UUGGcAGCUu--CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 11871 | 0.66 | 0.894932 |
Target: 5'- -gACGCCGAcucGAUCGcCGA-GUCCGc- -3' miRNA: 3'- agUGCGGCU---UUGGCaGCUuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 14230 | 0.66 | 0.902063 |
Target: 5'- gUCGCgGCC-AGGCaCGUCGAuGUCCGc- -3' miRNA: 3'- -AGUG-CGGcUUUG-GCAGCUuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 48237 | 0.67 | 0.846477 |
Target: 5'- aUCcCGUCGAAGCagaaGUCGAAcguGUCUggGg -3' miRNA: 3'- -AGuGCGGCUUUGg---CAGCUU---CAGGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 43403 | 0.67 | 0.863664 |
Target: 5'- --uCGCUGAGugCGUUGAuGUCUggGc -3' miRNA: 3'- aguGCGGCUUugGCAGCUuCAGGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 2092 | 0.67 | 0.855193 |
Target: 5'- gCACGCacaGAAGCUGUCGAAccuGUCg--- -3' miRNA: 3'- aGUGCGg--CUUUGGCAGCUU---CAGguuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 12743 | 0.67 | 0.863664 |
Target: 5'- uUCGgGCCG-GAUCGUCGcccGUCCGAc -3' miRNA: 3'- -AGUgCGGCuUUGGCAGCuu-CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 23845 | 0.67 | 0.846477 |
Target: 5'- -gGCGCUGggGCUGg-GAAGgCCAGGc -3' miRNA: 3'- agUGCGGCuuUGGCagCUUCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 8776 | 0.67 | 0.846477 |
Target: 5'- cCGCGCCGA---CGUgaaGGAGUUCGAGg -3' miRNA: 3'- aGUGCGGCUuugGCAg--CUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 30852 | 0.67 | 0.846477 |
Target: 5'- aUCuuGCCGggGagcuguCCGaCGAAGUCgGAGa -3' miRNA: 3'- -AGugCGGCuuU------GGCaGCUUCAGgUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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