Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7348 | 3' | -51.4 | NC_001900.1 | + | 37139 | 1.1 | 0.001983 |
Target: 5'- gUCACGCCGAAACCGUCGAAGUCCAAGa -3' miRNA: 3'- -AGUGCGGCUUUGGCAGCUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 14683 | 0.79 | 0.243481 |
Target: 5'- aUCACGCUGGAGCCGUgGGAGcCCu-- -3' miRNA: 3'- -AGUGCGGCUUUGGCAgCUUCaGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 39303 | 0.74 | 0.45195 |
Target: 5'- cCGCGCCGAGcCCGUgGggGUCg--- -3' miRNA: 3'- aGUGCGGCUUuGGCAgCuuCAGguuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 17241 | 0.74 | 0.48286 |
Target: 5'- cUCGC-CCGAGACC-UCGggGUcgCCAAGg -3' miRNA: 3'- -AGUGcGGCUUUGGcAGCuuCA--GGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 45620 | 0.73 | 0.514753 |
Target: 5'- --gUGUCGguGCCGUUGAGGUUCAGGg -3' miRNA: 3'- aguGCGGCuuUGGCAGCUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 27529 | 0.73 | 0.514753 |
Target: 5'- cUCACGCCGAGGaaGUCGGcGGUCUg-- -3' miRNA: 3'- -AGUGCGGCUUUggCAGCU-UCAGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 37154 | 0.73 | 0.525576 |
Target: 5'- uUCuuGUCGAAACCGcCGAucAGUUCGAGg -3' miRNA: 3'- -AGugCGGCUUUGGCaGCU--UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 27732 | 0.72 | 0.558524 |
Target: 5'- aCGCGCCGAAcaCGUCGGaguagagcgGGUUCAGGa -3' miRNA: 3'- aGUGCGGCUUugGCAGCU---------UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 18904 | 0.72 | 0.558524 |
Target: 5'- cCugGCCGAGAUgGUCcaGGAGgCCAAGa -3' miRNA: 3'- aGugCGGCUUUGgCAG--CUUCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 38747 | 0.71 | 0.603281 |
Target: 5'- gUCACGCC-AGACCGUCuccaugcgGAGcauGUCCGGGg -3' miRNA: 3'- -AGUGCGGcUUUGGCAG--------CUU---CAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 46619 | 0.71 | 0.603281 |
Target: 5'- aCGCaGCCGggGCCGUCGAccucacacAG-CCAc- -3' miRNA: 3'- aGUG-CGGCuuUGGCAGCU--------UCaGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 40924 | 0.71 | 0.614559 |
Target: 5'- uUCGCGCCGuagacGCCGUUGAugaucgCCAGGu -3' miRNA: 3'- -AGUGCGGCuu---UGGCAGCUuca---GGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 26884 | 0.71 | 0.614559 |
Target: 5'- gUCGCaacgGCCGGAACCGUCGccGUCg--- -3' miRNA: 3'- -AGUG----CGGCUUUGGCAGCuuCAGguuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 23487 | 0.71 | 0.625853 |
Target: 5'- cCACGCgGGggcagcGGCUGUCGAAGUCUg-- -3' miRNA: 3'- aGUGCGgCU------UUGGCAGCUUCAGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 26257 | 0.71 | 0.637153 |
Target: 5'- cUCGCGCuCG--GCCGgCGggGUCCAc- -3' miRNA: 3'- -AGUGCG-GCuuUGGCaGCuuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 48687 | 0.71 | 0.648448 |
Target: 5'- gUCACGCUGucGCUGUCGucG-CCGGGu -3' miRNA: 3'- -AGUGCGGCuuUGGCAGCuuCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 24551 | 0.71 | 0.659727 |
Target: 5'- aCGCGCCGAccGCCGUgacCGGAGUCa--- -3' miRNA: 3'- aGUGCGGCUu-UGGCA---GCUUCAGguuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 23351 | 0.71 | 0.659727 |
Target: 5'- aUCGuCGUCGGcaacaccaaGACCGUC-AGGUCCAAGc -3' miRNA: 3'- -AGU-GCGGCU---------UUGGCAGcUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 47220 | 0.7 | 0.670979 |
Target: 5'- gCAgGCCGAGGCCGUCacccGGcuuUCCGGGg -3' miRNA: 3'- aGUgCGGCUUUGGCAGcu--UC---AGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 24153 | 0.7 | 0.670979 |
Target: 5'- -aGCGCC---GCCGUCGuAGGUCCGGu -3' miRNA: 3'- agUGCGGcuuUGGCAGC-UUCAGGUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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