Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7348 | 3' | -51.4 | NC_001900.1 | + | 2092 | 0.67 | 0.855193 |
Target: 5'- gCACGCacaGAAGCUGUCGAAccuGUCg--- -3' miRNA: 3'- aGUGCGg--CUUUGGCAGCUU---CAGguuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 2972 | 0.68 | 0.789547 |
Target: 5'- uUCGCGgCGAAGCCGUUGGua--CGGGg -3' miRNA: 3'- -AGUGCgGCUUUGGCAGCUucagGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 3440 | 0.69 | 0.752251 |
Target: 5'- cCACGCUGGccuGGCCGUggcagaCGAcgaaguuucaggggaAGUCCAAGg -3' miRNA: 3'- aGUGCGGCU---UUGGCA------GCU---------------UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 4405 | 0.7 | 0.682192 |
Target: 5'- cUguCGCCGAGGCgGUCGAGGUaUggGa -3' miRNA: 3'- -AguGCGGCUUUGgCAGCUUCAgGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 4579 | 0.67 | 0.828341 |
Target: 5'- -aGCGCCGGuacACCGUCGGccuGGagCAGGu -3' miRNA: 3'- agUGCGGCUu--UGGCAGCU---UCagGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 5593 | 0.69 | 0.769068 |
Target: 5'- uUCAccCGCUGGAGCUGUCGGAGcugcgCUggGc -3' miRNA: 3'- -AGU--GCGGCUUUGGCAGCUUCa----GGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 8187 | 0.69 | 0.765942 |
Target: 5'- --uCGCCGAGAaggcguaCGUCGAguggcaggacgugcAGUCCGGGa -3' miRNA: 3'- aguGCGGCUUUg------GCAGCU--------------UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 8776 | 0.67 | 0.846477 |
Target: 5'- cCGCGCCGA---CGUgaaGGAGUUCGAGg -3' miRNA: 3'- aGUGCGGCUuugGCAg--CUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 9608 | 0.68 | 0.81799 |
Target: 5'- gUACGCCGAGAUCGauccucggaucggUCGGccGG-CCAAGg -3' miRNA: 3'- aGUGCGGCUUUGGC-------------AGCU--UCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 10969 | 0.66 | 0.894932 |
Target: 5'- -aACGCCGAGGCaagaCGuacGUCCAGGa -3' miRNA: 3'- agUGCGGCUUUGgca-GCuu-CAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 11871 | 0.66 | 0.894932 |
Target: 5'- -gACGCCGAcucGAUCGcCGA-GUCCGc- -3' miRNA: 3'- agUGCGGCU---UUGGCaGCUuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 12187 | 0.69 | 0.769068 |
Target: 5'- --uCGCCGAGGCCGagaagaccUCGAucGUCCAGc -3' miRNA: 3'- aguGCGGCUUUGGC--------AGCUu-CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 12743 | 0.67 | 0.863664 |
Target: 5'- uUCGgGCCG-GAUCGUCGcccGUCCGAc -3' miRNA: 3'- -AGUgCGGCuUUGGCAGCuu-CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 14230 | 0.66 | 0.902063 |
Target: 5'- gUCGCgGCC-AGGCaCGUCGAuGUCCGc- -3' miRNA: 3'- -AGUG-CGGcUUUG-GCAGCUuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 14683 | 0.79 | 0.243481 |
Target: 5'- aUCACGCUGGAGCCGUgGGAGcCCu-- -3' miRNA: 3'- -AGUGCGGCUUUGGCAgCUUCaGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15055 | 0.66 | 0.887521 |
Target: 5'- aUACGCCGGccgaacugaaGACCaUCGAccuGUCCGAc -3' miRNA: 3'- aGUGCGGCU----------UUGGcAGCUu--CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15825 | 0.66 | 0.894932 |
Target: 5'- cCGCGCCGugGCUGgugcCGAAGUacgCCAGc -3' miRNA: 3'- aGUGCGGCuuUGGCa---GCUUCA---GGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15829 | 0.69 | 0.747993 |
Target: 5'- ---gGUCGAGGCCGUCaagGAGGUCgAGGa -3' miRNA: 3'- agugCGGCUUUGGCAG---CUUCAGgUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 17241 | 0.74 | 0.48286 |
Target: 5'- cUCGC-CCGAGACC-UCGggGUcgCCAAGg -3' miRNA: 3'- -AGUGcGGCUUUGGcAGCuuCA--GGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 17470 | 0.66 | 0.901362 |
Target: 5'- ---gGCCGAAccgccagaagcucACCGagcgUGAGGUCCGGGa -3' miRNA: 3'- agugCGGCUU-------------UGGCa---GCUUCAGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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