Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7348 | 3' | -51.4 | NC_001900.1 | + | 8187 | 0.69 | 0.765942 |
Target: 5'- --uCGCCGAGAaggcguaCGUCGAguggcaggacgugcAGUCCGGGa -3' miRNA: 3'- aguGCGGCUUUg------GCAGCU--------------UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 12743 | 0.67 | 0.863664 |
Target: 5'- uUCGgGCCG-GAUCGUCGcccGUCCGAc -3' miRNA: 3'- -AGUgCGGCuUUGGCAGCuu-CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 45888 | 0.66 | 0.871881 |
Target: 5'- -gACGCUGAAG-CGUCG-GGUCCGc- -3' miRNA: 3'- agUGCGGCUUUgGCAGCuUCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 31517 | 0.66 | 0.887521 |
Target: 5'- uUCACGCCGuAGuuGUCGGcgAGaUCCu-- -3' miRNA: 3'- -AGUGCGGCuUUggCAGCU--UC-AGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15055 | 0.66 | 0.887521 |
Target: 5'- aUACGCCGGccgaacugaaGACCaUCGAccuGUCCGAc -3' miRNA: 3'- aGUGCGGCU----------UUGGcAGCUu--CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 11871 | 0.66 | 0.894932 |
Target: 5'- -gACGCCGAcucGAUCGcCGA-GUCCGc- -3' miRNA: 3'- agUGCGGCU---UUGGCaGCUuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 14230 | 0.66 | 0.902063 |
Target: 5'- gUCGCgGCC-AGGCaCGUCGAuGUCCGc- -3' miRNA: 3'- -AGUG-CGGcUUUG-GCAGCUuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 9608 | 0.68 | 0.81799 |
Target: 5'- gUACGCCGAGAUCGauccucggaucggUCGGccGG-CCAAGg -3' miRNA: 3'- aGUGCGGCUUUGGC-------------AGCU--UCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 12187 | 0.69 | 0.769068 |
Target: 5'- --uCGCCGAGGCCGagaagaccUCGAucGUCCAGc -3' miRNA: 3'- aguGCGGCUUUGGC--------AGCUu-CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 5593 | 0.69 | 0.769068 |
Target: 5'- uUCAccCGCUGGAGCUGUCGGAGcugcgCUggGc -3' miRNA: 3'- -AGU--GCGGCUUUGGCAGCUUCa----GGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 28010 | 0.65 | 0.906205 |
Target: 5'- aUCACGCCGuucgggccgguguAACCGggugCGGAG-CCGc- -3' miRNA: 3'- -AGUGCGGCu------------UUGGCa---GCUUCaGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15829 | 0.69 | 0.747993 |
Target: 5'- ---gGUCGAGGCCGUCaagGAGGUCgAGGa -3' miRNA: 3'- agugCGGCUUUGGCAG---CUUCAGgUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 37261 | 0.69 | 0.74157 |
Target: 5'- -gACGCCGAcagGGCCGUCGuacacggcccgcagcGGGgCCAGGa -3' miRNA: 3'- agUGCGGCU---UUGGCAGC---------------UUCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 19545 | 0.7 | 0.704457 |
Target: 5'- aCAcCGCCGugGCCGUgGGAGgaacugcCCAAGg -3' miRNA: 3'- aGU-GCGGCuuUGGCAgCUUCa------GGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 48687 | 0.71 | 0.648448 |
Target: 5'- gUCACGCUGucGCUGUCGucG-CCGGGu -3' miRNA: 3'- -AGUGCGGCuuUGGCAGCuuCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 26257 | 0.71 | 0.637153 |
Target: 5'- cUCGCGCuCG--GCCGgCGggGUCCAc- -3' miRNA: 3'- -AGUGCG-GCuuUGGCaGCuuCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 40924 | 0.71 | 0.614559 |
Target: 5'- uUCGCGCCGuagacGCCGUUGAugaucgCCAGGu -3' miRNA: 3'- -AGUGCGGCuu---UGGCAGCUuca---GGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 26884 | 0.71 | 0.614559 |
Target: 5'- gUCGCaacgGCCGGAACCGUCGccGUCg--- -3' miRNA: 3'- -AGUG----CGGCUUUGGCAGCuuCAGguuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 27529 | 0.73 | 0.514753 |
Target: 5'- cUCACGCCGAGGaaGUCGGcGGUCUg-- -3' miRNA: 3'- -AGUGCGGCUUUggCAGCU-UCAGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 37139 | 1.1 | 0.001983 |
Target: 5'- gUCACGCCGAAACCGUCGAAGUCCAAGa -3' miRNA: 3'- -AGUGCGGCUUUGGCAGCUUCAGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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