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miRNA: 3'- guGCCCUU----UCGAGuUGCCGGCA----UGCG- -5'
miRNA: 3'- -GUGCCCuu-UCGAGuuGCCGGCAuGCG- -5'
miRNA: 3'- gUGCCCUU----UC-GAGUUGCCG--GCAugCG- -5'
miRNA: 3'- gugCCCU---UUCGAGUUGCCGGCaUGCG- -5'
miRNA: 3'- -GUGCCCUUUCGAGUUGCCGGCAUGCG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 8083 | 5' | -54 | NC_001978.2 | + | 18458 | 0.76 | 0.219394 |
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Target: 5'- -cCGGGAAguccGGCUUcACGGCCGUagcgACGCc -3' miRNA: 3'- guGCCCUU----UCGAGuUGCCGGCA----UGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 13875 | 0.76 | 0.213537 |
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Target: 5'- cCACGGGugcGGCUC--CGGCCGU-CGCu -3' miRNA: 3'- -GUGCCCuu-UCGAGuuGCCGGCAuGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 257 | 0.77 | 0.176202 |
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Target: 5'- gACGGGGGcagcGGaCUCAGCGGCagCGUugGCg -3' miRNA: 3'- gUGCCCUU----UC-GAGUUGCCG--GCAugCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 4996 | 0.81 | 0.099683 |
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Target: 5'- --aGGGAccgAAGCUCAGCGGUCGcACGCu -3' miRNA: 3'- gugCCCU---UUCGAGUUGCCGGCaUGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 7593 | 1.12 | 0.000591 |
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Target: 5'- gCACGGGAAAGCUCAACGGCCGUACGCg -3' miRNA: 3'- -GUGCCCUUUCGAGUUGCCGGCAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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