Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8083 | 5' | -54 | NC_001978.2 | + | 23595 | 0.66 | 0.70448 |
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Target: 5'- uCACGGuGAuugccGGGUUCGGCGcGUCGUACu- -3' miRNA: 3'- -GUGCC-CU-----UUCGAGUUGC-CGGCAUGcg -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 9771 | 0.66 | 0.726396 |
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Target: 5'- aCGCGuugcuGAAGCUCGAcccgucguCGGCCGUugagACGCu -3' miRNA: 3'- -GUGCcc---UUUCGAGUU--------GCCGGCA----UGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 39447 | 0.66 | 0.733979 |
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Target: 5'- aGCGGGcucgauGAucgguccgcaaucaAGCUCGAcCGGCCGgaGCGUg -3' miRNA: 3'- gUGCCC------UU--------------UCGAGUU-GCCGGCa-UGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 10693 | 0.66 | 0.737213 |
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Target: 5'- aACGGucuuucGAAGCUgGGCGGCUuUGCGUc -3' miRNA: 3'- gUGCCc-----UUUCGAgUUGCCGGcAUGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 13293 | 0.66 | 0.737213 |
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Target: 5'- gGCGGGu--GCUCcauGACGGguaCGaGCGCa -3' miRNA: 3'- gUGCCCuuuCGAG---UUGCCg--GCaUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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