Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8083 | 5' | -54 | NC_001978.2 | + | 38229 | 0.72 | 0.384973 |
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Target: 5'- gCACGGGGAAGCcgcgccacgccuUCGggacgucaGCGGCucgcccCGUGCGCu -3' miRNA: 3'- -GUGCCCUUUCG------------AGU--------UGCCG------GCAUGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 38910 | 0.67 | 0.626057 |
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Target: 5'- gGCGGGAAGGCgcuuACGGCUucgaGCu -3' miRNA: 3'- gUGCCCUUUCGagu-UGCCGGcaugCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 39447 | 0.66 | 0.733979 |
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Target: 5'- aGCGGGcucgauGAucgguccgcaaucaAGCUCGAcCGGCCGgaGCGUg -3' miRNA: 3'- gUGCCC------UU--------------UCGAGUU-GCCGGCa-UGCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 39838 | 0.68 | 0.592309 |
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Target: 5'- cCGCGcGGcAGGCgcguaCGACGGacCCGUugGCa -3' miRNA: 3'- -GUGC-CCuUUCGa----GUUGCC--GGCAugCG- -5' |
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| 8083 | 5' | -54 | NC_001978.2 | + | 41409 | 0.67 | 0.647474 |
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Target: 5'- gGCGGGgcGGCUCcgaaguuGGCGGCgUGUGCc- -3' miRNA: 3'- gUGCCCuuUCGAG-------UUGCCG-GCAUGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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