Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8094 | 3' | -56.4 | NC_001978.2 | + | 16693 | 0.66 | 0.659999 |
Target: 5'- uGCGGucaACGcauGUCGGCGCCGuuCCGc -3' miRNA: 3'- gCGCU---UGCcu-CAGCUGCGGCuuGGCc -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 27940 | 0.66 | 0.649157 |
Target: 5'- aGuCGAACGGGgcGUUGugGaucagGAACCGGg -3' miRNA: 3'- gC-GCUUGCCU--CAGCugCgg---CUUGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 4494 | 0.66 | 0.649157 |
Target: 5'- aGgGAACGcGuGUCGugGuuGAACgUGGc -3' miRNA: 3'- gCgCUUGC-CuCAGCugCggCUUG-GCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 21778 | 0.66 | 0.648071 |
Target: 5'- gGUG-ACGuAGUgaugaagUGGCGUCGAGCCGGg -3' miRNA: 3'- gCGCuUGCcUCA-------GCUGCGGCUUGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 12636 | 0.66 | 0.638299 |
Target: 5'- uCGUGAAccaaucgcCGGAGUCGA-GCCGcACCc- -3' miRNA: 3'- -GCGCUU--------GCCUCAGCUgCGGCuUGGcc -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 7976 | 0.66 | 0.638299 |
Target: 5'- aCGUGAgcuugauuGCGGGcGUC-ACGCCGucgcCCGGg -3' miRNA: 3'- -GCGCU--------UGCCU-CAGcUGCGGCuu--GGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 33933 | 0.66 | 0.638299 |
Target: 5'- gGCGAccgGCGaaGAGaucaCGGCGCUucuGAACCGGg -3' miRNA: 3'- gCGCU---UGC--CUCa---GCUGCGG---CUUGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 30042 | 0.66 | 0.631782 |
Target: 5'- uCGCGcucAGCGGcaagccggucggcaaGGUCGuCGgCGGACUGGg -3' miRNA: 3'- -GCGC---UUGCC---------------UCAGCuGCgGCUUGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 28947 | 0.66 | 0.627437 |
Target: 5'- gCGCGAcCGGAagguGUUcaagGGCGCCGGAuUCGGc -3' miRNA: 3'- -GCGCUuGCCU----CAG----CUGCGGCUU-GGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 39403 | 0.66 | 0.627437 |
Target: 5'- gCGUGAuCGG-GUCG-CGCUGAAUgCGGu -3' miRNA: 3'- -GCGCUuGCCuCAGCuGCGGCUUG-GCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 5497 | 0.66 | 0.627437 |
Target: 5'- cCGCccaugGAACGcuGAGUCGugGCCGggUagcucucaGGa -3' miRNA: 3'- -GCG-----CUUGC--CUCAGCugCGGCuuGg-------CC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 20387 | 0.66 | 0.627437 |
Target: 5'- gGCGAAgGccgaagucGAGUCG-CGCCGGucgGCCGa -3' miRNA: 3'- gCGCUUgC--------CUCAGCuGCGGCU---UGGCc -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 1105 | 0.66 | 0.627437 |
Target: 5'- aCGCGGACGuAGugaaggccguUCGucuUGCCGAaguACCGGg -3' miRNA: 3'- -GCGCUUGCcUC----------AGCu--GCGGCU---UGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 38027 | 0.66 | 0.627437 |
Target: 5'- gCGCGcugccccaGGAcGaCGACGgCGAGCCGGu -3' miRNA: 3'- -GCGCuug-----CCU-CaGCUGCgGCUUGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 28039 | 0.66 | 0.627437 |
Target: 5'- uGCGuucuACGucGAcccGUCGGCGCCGGACacucaGGg -3' miRNA: 3'- gCGCu---UGC--CU---CAGCUGCGGCUUGg----CC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 25664 | 0.66 | 0.627437 |
Target: 5'- gGCGuguucCGGGGU-GACGCCGGuuaCGGc -3' miRNA: 3'- gCGCuu---GCCUCAgCUGCGGCUug-GCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 13947 | 0.66 | 0.616578 |
Target: 5'- uCGUGAuuACGGAGcgcUCGACGUCGuugaugagaacGGCaCGGa -3' miRNA: 3'- -GCGCU--UGCCUC---AGCUGCGGC-----------UUG-GCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 3120 | 0.66 | 0.616578 |
Target: 5'- uCGCGuaaGGAGauagUGACGCCGGAauGGc -3' miRNA: 3'- -GCGCuugCCUCa---GCUGCGGCUUggCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 34713 | 0.66 | 0.613323 |
Target: 5'- gGUGcAACGGggcucAGUCGGCGUCGGcucgaacccggcaaGCUGGg -3' miRNA: 3'- gCGC-UUGCC-----UCAGCUGCGGCU--------------UGGCC- -5' |
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8094 | 3' | -56.4 | NC_001978.2 | + | 5999 | 0.67 | 0.605733 |
Target: 5'- gCGCGccGACGGucuucuuGUCGACGCCcgGGGCgCGa -3' miRNA: 3'- -GCGC--UUGCCu------CAGCUGCGG--CUUG-GCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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