Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8207 | 5' | -55.6 | NC_001978.2 | + | 33134 | 0.72 | 0.304586 |
Target: 5'- gGCaaGCGUUGGCaCGuugcCGCCGACGCUGAg -3' miRNA: 3'- gCGa-UGCAGCUG-GU----GCGGCUGUGACU- -5' |
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8207 | 5' | -55.6 | NC_001978.2 | + | 9006 | 0.72 | 0.297043 |
Target: 5'- gCGCUAucgcCGUCGGCauugGCGCCGGuauCGCUGAg -3' miRNA: 3'- -GCGAU----GCAGCUGg---UGCGGCU---GUGACU- -5' |
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8207 | 5' | -55.6 | NC_001978.2 | + | 41278 | 0.74 | 0.241883 |
Target: 5'- aGCUcgACGUCGACCACaucaaGCCGcuuGCAUUGGg -3' miRNA: 3'- gCGA--UGCAGCUGGUG-----CGGC---UGUGACU- -5' |
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8207 | 5' | -55.6 | NC_001978.2 | + | 28044 | 0.75 | 0.206333 |
Target: 5'- -uCUACGUCGACC-CGUCGGCGCcgGAc -3' miRNA: 3'- gcGAUGCAGCUGGuGCGGCUGUGa-CU- -5' |
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8207 | 5' | -55.6 | NC_001978.2 | + | 12390 | 0.77 | 0.140596 |
Target: 5'- uGCcgaagGCGUCGACCACGUUcACGCUGAc -3' miRNA: 3'- gCGa----UGCAGCUGGUGCGGcUGUGACU- -5' |
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8207 | 5' | -55.6 | NC_001978.2 | + | 32888 | 1.09 | 0.000729 |
Target: 5'- gCGCUACGUCGACCACGCCGACACUGAg -3' miRNA: 3'- -GCGAUGCAGCUGGUGCGGCUGUGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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