Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8491 | 5' | -57.8 | NC_002169.1 | + | 40184 | 0.66 | 0.802235 |
Target: 5'- aUGGCCaucaguguggugaUCGAucCCGUCAcguacaucaacauuaGCGUCGCCAa -3' miRNA: 3'- aAUCGG-------------AGCU--GGCAGU---------------UGCGGCGGUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 107698 | 0.66 | 0.799536 |
Target: 5'- -aAGUCUCGACCaucGUCGGCcaccaaUGCCGCg -3' miRNA: 3'- aaUCGGAGCUGG---CAGUUGcg----GCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 55393 | 0.66 | 0.79044 |
Target: 5'- uUUGGCgUCGAgauauagugaaUCGUCGACGUCGCgGa -3' miRNA: 3'- -AAUCGgAGCU-----------GGCAGUUGCGGCGgUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 22362 | 0.66 | 0.79044 |
Target: 5'- -cGGCCagcacgugcCGGCCGgaaagauugcgcUCGGCGUCGCCAUg -3' miRNA: 3'- aaUCGGa--------GCUGGC------------AGUUGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 128106 | 0.66 | 0.7812 |
Target: 5'- --cGCCgCcGCCGcCGcCGCCGCCGCc -3' miRNA: 3'- aauCGGaGcUGGCaGUuGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 125102 | 0.66 | 0.771824 |
Target: 5'- --uGUCUCacacuCUGUCGcCGCCGCCGCc -3' miRNA: 3'- aauCGGAGcu---GGCAGUuGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 116099 | 0.66 | 0.771824 |
Target: 5'- gUAGUCgaauaugucgCGGCCGUCu-CGCuUGCCGCg -3' miRNA: 3'- aAUCGGa---------GCUGGCAGuuGCG-GCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 118741 | 0.66 | 0.771824 |
Target: 5'- -aGGCUcucaUCGAUCGggUCAACGgCGCCAa -3' miRNA: 3'- aaUCGG----AGCUGGC--AGUUGCgGCGGUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 91872 | 0.66 | 0.762322 |
Target: 5'- ----aCUUGACUGUCGacgacgACGCCGUCGCc -3' miRNA: 3'- aaucgGAGCUGGCAGU------UGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 6223 | 0.66 | 0.762322 |
Target: 5'- gUAGUCgCcGCCGcCGcCGCCGCCGCc -3' miRNA: 3'- aAUCGGaGcUGGCaGUuGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 38000 | 0.66 | 0.762322 |
Target: 5'- --cGgCUCGACUGUaCAACaaaGCCGCgGCa -3' miRNA: 3'- aauCgGAGCUGGCA-GUUG---CGGCGgUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 118276 | 0.67 | 0.752703 |
Target: 5'- -gAGCCggCGGCguaaUCAAUGCCGCCGu -3' miRNA: 3'- aaUCGGa-GCUGgc--AGUUGCGGCGGUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 53443 | 0.67 | 0.74102 |
Target: 5'- -aAGCCggucgggaaacgCGGCCaaguaucagGUCGuCGCCGCCGCc -3' miRNA: 3'- aaUCGGa-----------GCUGG---------CAGUuGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 48127 | 0.67 | 0.733153 |
Target: 5'- --uGUCUCauacaaaccGCCGUCGGCGCCGUCGu -3' miRNA: 3'- aauCGGAGc--------UGGCAGUUGCGGCGGUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 108888 | 0.67 | 0.733153 |
Target: 5'- --cGUCgUCG-UCGUCGcCGCCGCCGCc -3' miRNA: 3'- aauCGG-AGCuGGCAGUuGCGGCGGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 36941 | 0.67 | 0.723239 |
Target: 5'- -cGGCUacgUGGCCGacuUCAACGCCGaCACg -3' miRNA: 3'- aaUCGGa--GCUGGC---AGUUGCGGCgGUG- -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 56990 | 0.68 | 0.682886 |
Target: 5'- --cGCCgUGGCCGcuuUgGACGCCGCCGg -3' miRNA: 3'- aauCGGaGCUGGC---AgUUGCGGCGGUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 120446 | 0.68 | 0.682886 |
Target: 5'- -aAGCC-CGACaccauauacguCGUCAACGuuGCCGa -3' miRNA: 3'- aaUCGGaGCUG-----------GCAGUUGCggCGGUg -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 110408 | 0.68 | 0.662418 |
Target: 5'- --cGCCcuuUUGGCCGUgAACGCCGCa-- -3' miRNA: 3'- aauCGG---AGCUGGCAgUUGCGGCGgug -5' |
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8491 | 5' | -57.8 | NC_002169.1 | + | 100403 | 0.69 | 0.641852 |
Target: 5'- -cGGUaucgUUCaGACCGUCGGCGCUaaagGCCACg -3' miRNA: 3'- aaUCG----GAG-CUGGCAGUUGCGG----CGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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