Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8808 | 5' | -60.8 | NC_002484.1 | + | 13479 | 0.7 | 0.228063 |
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Target: 5'- cGGCA--GCCGAa-GCAGCCgCCGCCg -3' miRNA: 3'- aCCGUcgCGGUUagCGUCGGgGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 904 | 0.7 | 0.228063 |
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Target: 5'- gUGGUGGCGCCGgaGUagaccuCGGCCCCCgacucGCCa -3' miRNA: 3'- -ACCGUCGCGGU--UAgc----GUCGGGGG-----UGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 42202 | 0.7 | 0.222321 |
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Target: 5'- gGGaCcGCGCCGA-CGCcGCCCUgGCCa -3' miRNA: 3'- aCC-GuCGCGGUUaGCGuCGGGGgUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 43389 | 0.71 | 0.195431 |
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Target: 5'- -aGCAGCacuaGCCAAcCGCaAGCCUCCAUCa -3' miRNA: 3'- acCGUCG----CGGUUaGCG-UCGGGGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 4032 | 0.74 | 0.11421 |
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Target: 5'- aGGCGGCgcgGCCGAU-GCGGauuucauCCCCCGCCa -3' miRNA: 3'- aCCGUCG---CGGUUAgCGUC-------GGGGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 26110 | 0.76 | 0.079992 |
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Target: 5'- cGGUAGUGCCGcgCGCcgAGCCagacaCCGCCg -3' miRNA: 3'- aCCGUCGCGGUuaGCG--UCGGg----GGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 11494 | 1.11 | 0.000198 |
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Target: 5'- gUGGCAGCGCCAAUCGCAGCCCCCACCu -3' miRNA: 3'- -ACCGUCGCGGUUAGCGUCGGGGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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