Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8901 | 3' | -53.5 | NC_002484.1 | + | 26504 | 0.7 | 0.609272 |
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Target: 5'- aCCUcgacCGCgGGCGcucgcacggCACAGCAAUGGgCa -3' miRNA: 3'- -GGAu---GCGgCCGCa--------GUGUUGUUACCgG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 6941 | 0.7 | 0.608172 |
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Target: 5'- gCCUGCGUa-GCGUcCGCAGCGAUcaacucgcgacccGGCCc -3' miRNA: 3'- -GGAUGCGgcCGCA-GUGUUGUUA-------------CCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 4745 | 0.71 | 0.56555 |
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Target: 5'- uCCU-CGCCGcCGUCACGcuucuCGuUGGCCa -3' miRNA: 3'- -GGAuGCGGCcGCAGUGUu----GUuACCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 51884 | 0.71 | 0.54398 |
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Target: 5'- cCUUACGCgccuugaucuccCGGCGguugCGguCGGCGAUGGCCu -3' miRNA: 3'- -GGAUGCG------------GCCGCa---GU--GUUGUUACCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 42758 | 0.72 | 0.470991 |
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Target: 5'- gCCUGcCGCCGGUGggCACGcuguGCgAGUGGCa -3' miRNA: 3'- -GGAU-GCGGCCGCa-GUGU----UG-UUACCGg -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 33887 | 0.73 | 0.460962 |
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Target: 5'- gCUACGUCGGaGUcCACAACAGcauUGGCg -3' miRNA: 3'- gGAUGCGGCCgCA-GUGUUGUU---ACCGg -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 53966 | 0.74 | 0.394151 |
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Target: 5'- aCUA-GCCGGCGUCACGcccgcagaGCAagGaGCCg -3' miRNA: 3'- gGAUgCGGCCGCAGUGU--------UGUuaC-CGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 39383 | 0.89 | 0.04211 |
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Target: 5'- aCCUagagcgGCGCUGGUGUCACGuuuGCAAUGGCCg -3' miRNA: 3'- -GGA------UGCGGCCGCAGUGU---UGUUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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