Results 1 - 20 of 55 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8949 | 5' | -59.4 | NC_002512.2 | + | 95043 | 0.66 | 0.817708 |
Target: 5'- -gGCUGUgCguguaguagGCCUCCGCCGucGGCa- -3' miRNA: 3'- gaCGACAgG---------UGGAGGCGGCucCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 77065 | 0.66 | 0.817708 |
Target: 5'- gCUGCUGaUCCugCUCgccgcccgccguCGCCGcGGACc- -3' miRNA: 3'- -GACGAC-AGGugGAG------------GCGGCuCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 174334 | 0.66 | 0.817708 |
Target: 5'- -gGCggggGUCCGCCgcgcccucgUCCGCCG-GGAUg- -3' miRNA: 3'- gaCGa---CAGGUGG---------AGGCGGCuCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 176614 | 0.66 | 0.825934 |
Target: 5'- gUGUcGaucUCCACC-CCGUCGGGGGCUc -3' miRNA: 3'- gACGaC---AGGUGGaGGCGGCUCCUGAa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 75128 | 0.66 | 0.833996 |
Target: 5'- gCUGCUGgugguggUCACCgCCaCCGGGGGCg- -3' miRNA: 3'- -GACGACa------GGUGGaGGcGGCUCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 213032 | 0.66 | 0.833996 |
Target: 5'- gUGC-GUCCGCCggCUGCgGGGGAUc- -3' miRNA: 3'- gACGaCAGGUGGa-GGCGgCUCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 145903 | 0.66 | 0.849605 |
Target: 5'- gCUGCgcgaCCGCCccgcgccgggcaUCCGCCGccGGGACUUc -3' miRNA: 3'- -GACGaca-GGUGG------------AGGCGGC--UCCUGAA- -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 145440 | 0.66 | 0.825934 |
Target: 5'- gUGCUGgacgagauccUCCGCCUCaacgacaaGaCCGAGGACc- -3' miRNA: 3'- gACGAC----------AGGUGGAGg-------C-GGCUCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 34283 | 0.66 | 0.817708 |
Target: 5'- -aGgUGgCCGCC-CCGCCGAGGugACUg -3' miRNA: 3'- gaCgACaGGUGGaGGCGGCUCC--UGAa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 31466 | 0.66 | 0.833996 |
Target: 5'- cCUGCUGggCCACCUCgGa-GAcGGGCUg -3' miRNA: 3'- -GACGACa-GGUGGAGgCggCU-CCUGAa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 224911 | 0.66 | 0.849605 |
Target: 5'- -cGCcGUCUgcACCUCCGUCGcgGGGACc- -3' miRNA: 3'- gaCGaCAGG--UGGAGGCGGC--UCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 81794 | 0.66 | 0.841889 |
Target: 5'- -gGCg--CCGCCgccgCCGCCGgGGGACg- -3' miRNA: 3'- gaCGacaGGUGGa---GGCGGC-UCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 101401 | 0.66 | 0.841889 |
Target: 5'- -cGCUGUCCGCCgaCCGCUGucG-CUg -3' miRNA: 3'- gaCGACAGGUGGa-GGCGGCucCuGAa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 150018 | 0.66 | 0.825934 |
Target: 5'- -gGCUcUCUuCCUCCuCCGAGGACg- -3' miRNA: 3'- gaCGAcAGGuGGAGGcGGCUCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 106035 | 0.67 | 0.812697 |
Target: 5'- gUGCggcGUCC-UCUCCgccacgcagaucacgGCCGGGGACUUg -3' miRNA: 3'- gACGa--CAGGuGGAGG---------------CGGCUCCUGAA- -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 4716 | 0.67 | 0.800795 |
Target: 5'- aCUGCUG-CUGCUgcaacgCCGCCGAcGACUg -3' miRNA: 3'- -GACGACaGGUGGa-----GGCGGCUcCUGAa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 128552 | 0.67 | 0.791248 |
Target: 5'- aUGUcacaUGUCCACCUUCGacggccuCCGGGGGCc- -3' miRNA: 3'- gACG----ACAGGUGGAGGC-------GGCUCCUGaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 101446 | 0.67 | 0.783318 |
Target: 5'- cCUGCUGggCCAUggCCGCCcgcgagGAGGGCUc -3' miRNA: 3'- -GACGACa-GGUGgaGGCGG------CUCCUGAa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 12049 | 0.67 | 0.783318 |
Target: 5'- -cGCUG-CCGCCUCUGCCGAa----- -3' miRNA: 3'- gaCGACaGGUGGAGGCGGCUccugaa -5' |
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8949 | 5' | -59.4 | NC_002512.2 | + | 77166 | 0.67 | 0.765338 |
Target: 5'- uCUGCgUGUCCccugcccgccgcACC-CCGaCCGGGGACg- -3' miRNA: 3'- -GACG-ACAGG------------UGGaGGC-GGCUCCUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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