Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8955 | 3' | -56.8 | NC_002512.2 | + | 116889 | 0.66 | 0.951539 |
Target: 5'- gGCUGGCGGCGGggcCGGgGCgggcUCcUCGg -3' miRNA: 3'- gCGACCGCCGCU---GUCaCGau--AGcAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 212154 | 0.66 | 0.951539 |
Target: 5'- gGCgGGCacuGGCGGCGGgacgGCUGggcCGUCc -3' miRNA: 3'- gCGaCCG---CCGCUGUCa---CGAUa--GCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 80643 | 0.66 | 0.947497 |
Target: 5'- cCGUccGCGGCGGCGGcGCcggGUcCGUCGg -3' miRNA: 3'- -GCGacCGCCGCUGUCaCGa--UA-GCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 117435 | 0.66 | 0.947497 |
Target: 5'- aCGCUGGCcGUGAugcacCAGaaGCUGUCGcCGg -3' miRNA: 3'- -GCGACCGcCGCU-----GUCa-CGAUAGCaGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 219021 | 0.66 | 0.947081 |
Target: 5'- uGCU-GCuGCGAC-GUGCUggucuacGUCGUCGg -3' miRNA: 3'- gCGAcCGcCGCUGuCACGA-------UAGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 131679 | 0.66 | 0.946242 |
Target: 5'- cCGCgagGGCGGUGAgcccggagcgcaggUAGUGCUGcUCGgagaCGa -3' miRNA: 3'- -GCGa--CCGCCGCU--------------GUCACGAU-AGCa---GC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 92009 | 0.66 | 0.943238 |
Target: 5'- uCGCcGGCGGCGGacucGCgggCGUCGg -3' miRNA: 3'- -GCGaCCGCCGCUgucaCGauaGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 64798 | 0.66 | 0.93876 |
Target: 5'- uGCUGGCGccaGCGACGG-GCa--CGUCc -3' miRNA: 3'- gCGACCGC---CGCUGUCaCGauaGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 15328 | 0.66 | 0.93876 |
Target: 5'- uCGCccUGGUcgcGGUGACAGUGCUcgCaGUUa -3' miRNA: 3'- -GCG--ACCG---CCGCUGUCACGAuaG-CAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 89233 | 0.66 | 0.93876 |
Target: 5'- uGUUGGUgacGGCGACGGaGCUcUCG-CGg -3' miRNA: 3'- gCGACCG---CCGCUGUCaCGAuAGCaGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 81980 | 0.66 | 0.934062 |
Target: 5'- gGgUGGUGGCGGCGGggGCgucUCGggggCGg -3' miRNA: 3'- gCgACCGCCGCUGUCa-CGau-AGCa---GC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 146643 | 0.66 | 0.934062 |
Target: 5'- uGCUGGCGGCGACgAGgagggagGCU-UCccCGg -3' miRNA: 3'- gCGACCGCCGCUG-UCa------CGAuAGcaGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 22577 | 0.66 | 0.934062 |
Target: 5'- uGCUGGUgaacggcucggaGGCcACGGUGCUGgacaCGUCc -3' miRNA: 3'- gCGACCG------------CCGcUGUCACGAUa---GCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 75352 | 0.66 | 0.929142 |
Target: 5'- gCGgaGGCGGCGGacCGGUaGCgaucuUCGUCc -3' miRNA: 3'- -GCgaCCGCCGCU--GUCA-CGau---AGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 56303 | 0.66 | 0.929142 |
Target: 5'- uGCUGGCGGCGcCGGaGCUcggCG-Ca -3' miRNA: 3'- gCGACCGCCGCuGUCaCGAua-GCaGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 179485 | 0.67 | 0.924 |
Target: 5'- uGCUucGGCGGCGuCGucGCcAUCGUCGu -3' miRNA: 3'- gCGA--CCGCCGCuGUcaCGaUAGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 71140 | 0.67 | 0.924 |
Target: 5'- aGCagGGCGGCcAC-GUGUUccugGUCGUCGa -3' miRNA: 3'- gCGa-CCGCCGcUGuCACGA----UAGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 179563 | 0.67 | 0.918636 |
Target: 5'- gGCUcgaucuucGGCGGCGGCAGgaucGCaucuugggCGUCGa -3' miRNA: 3'- gCGA--------CCGCCGCUGUCa---CGaua-----GCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 161712 | 0.67 | 0.918636 |
Target: 5'- aCGC-GGcCGGCGACGGg---AUCGUCu -3' miRNA: 3'- -GCGaCC-GCCGCUGUCacgaUAGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 88273 | 0.67 | 0.918636 |
Target: 5'- ---aGGUGGCGGCAG-GCg--UGUCGg -3' miRNA: 3'- gcgaCCGCCGCUGUCaCGauaGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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