Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8955 | 3' | -56.8 | NC_002512.2 | + | 6407 | 0.69 | 0.845539 |
Target: 5'- gGgaGGCGGCGGCGGcGCUGgggaaggcggcCGUCu -3' miRNA: 3'- gCgaCCGCCGCUGUCaCGAUa----------GCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 12390 | 0.68 | 0.860647 |
Target: 5'- uCGCcuucgGGCGGCGGCGGcgGCgccgggAUCG-CGa -3' miRNA: 3'- -GCGa----CCGCCGCUGUCa-CGa-----UAGCaGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 15328 | 0.66 | 0.93876 |
Target: 5'- uCGCccUGGUcgcGGUGACAGUGCUcgCaGUUa -3' miRNA: 3'- -GCG--ACCG---CCGCUGUCACGAuaG-CAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 20299 | 0.67 | 0.907244 |
Target: 5'- aCG-UGGCGGUGACcGUGCcGgacgaggagcUCGUCGa -3' miRNA: 3'- -GCgACCGCCGCUGuCACGaU----------AGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 22577 | 0.66 | 0.934062 |
Target: 5'- uGCUGGUgaacggcucggaGGCcACGGUGCUGgacaCGUCc -3' miRNA: 3'- gCGACCG------------CCGcUGUCACGAUa---GCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 22992 | 0.67 | 0.907244 |
Target: 5'- aCGCgGGCGGCGcUGGUGacCUAccCGUCGg -3' miRNA: 3'- -GCGaCCGCCGCuGUCAC--GAUa-GCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 26406 | 0.68 | 0.860647 |
Target: 5'- uGCUGGCGaCG-CAGaUGCUGUCGa-- -3' miRNA: 3'- gCGACCGCcGCuGUC-ACGAUAGCagc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 26700 | 0.75 | 0.518337 |
Target: 5'- uGCUGGCGGUGACcugGCUggCGUCc -3' miRNA: 3'- gCGACCGCCGCUGucaCGAuaGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 33195 | 0.7 | 0.787343 |
Target: 5'- gGCUGGCGG--ACGGUGCcgcUCGUCc -3' miRNA: 3'- gCGACCGCCgcUGUCACGau-AGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 34627 | 0.69 | 0.837711 |
Target: 5'- aGCUGuaacGCGGCGAgacaCAGgagGCU-UCGUCGg -3' miRNA: 3'- gCGAC----CGCCGCU----GUCa--CGAuAGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 36441 | 0.68 | 0.881859 |
Target: 5'- cCGCUcccgccaccGGCGGCcGCGGUcgcGCUcguaGUCGUCGu -3' miRNA: 3'- -GCGA---------CCGCCGcUGUCA---CGA----UAGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 47015 | 0.67 | 0.907244 |
Target: 5'- uGCUGGCGGCGG-AGcUGCUGggCGa-- -3' miRNA: 3'- gCGACCGCCGCUgUC-ACGAUa-GCagc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 51928 | 0.68 | 0.887208 |
Target: 5'- aCGCUGGCGG-GACugaucacccaGCUGUCGgacgCGg -3' miRNA: 3'- -GCGACCGCCgCUGuca-------CGAUAGCa---GC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 56303 | 0.66 | 0.929142 |
Target: 5'- uGCUGGCGGCGcCGGaGCUcggCG-Ca -3' miRNA: 3'- gCGACCGCCGCuGUCaCGAua-GCaGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 64798 | 0.66 | 0.93876 |
Target: 5'- uGCUGGCGccaGCGACGG-GCa--CGUCc -3' miRNA: 3'- gCGACCGC---CGCUGUCaCGauaGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 71140 | 0.67 | 0.924 |
Target: 5'- aGCagGGCGGCcAC-GUGUUccugGUCGUCGa -3' miRNA: 3'- gCGa-CCGCCGcUGuCACGA----UAGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 75288 | 0.69 | 0.813214 |
Target: 5'- gGCggcGGCGGCGACGaUGCcgcCGUCGc -3' miRNA: 3'- gCGa--CCGCCGCUGUcACGauaGCAGC- -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 75352 | 0.66 | 0.929142 |
Target: 5'- gCGgaGGCGGCGGacCGGUaGCgaucuUCGUCc -3' miRNA: 3'- -GCgaCCGCCGCU--GUCA-CGau---AGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 79821 | 0.68 | 0.867917 |
Target: 5'- gGC-GGCGGCGGCGGUccccccucccGCcccGUCGUCc -3' miRNA: 3'- gCGaCCGCCGCUGUCA----------CGa--UAGCAGc -5' |
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8955 | 3' | -56.8 | NC_002512.2 | + | 80643 | 0.66 | 0.947497 |
Target: 5'- cCGUccGCGGCGGCGGcGCcggGUcCGUCGg -3' miRNA: 3'- -GCGacCGCCGCUGUCaCGa--UA-GCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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