Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8960 | 3' | -56.6 | NC_002512.2 | + | 218305 | 0.7 | 0.756481 |
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Target: 5'- cGGCCCUgggCGGCCGC-UCGuGCUCc- -3' miRNA: 3'- cUCGGGAa--GCUGGUGcAGCuCGAGuu -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 193187 | 0.7 | 0.756481 |
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Target: 5'- -cGCCuucuuCUUCGACCGCGUCuucGGCUCGc -3' miRNA: 3'- cuCGG-----GAAGCUGGUGCAGc--UCGAGUu -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 10808 | 0.7 | 0.747045 |
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Target: 5'- -cGUCCa-UGAgCACGUCGAGCUCGAa -3' miRNA: 3'- cuCGGGaaGCUgGUGCAGCUCGAGUU- -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 216863 | 0.71 | 0.727891 |
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Target: 5'- -cGCCCUcgUCGuCCGCGUCGucGUUCAGg -3' miRNA: 3'- cuCGGGA--AGCuGGUGCAGCu-CGAGUU- -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 140565 | 0.71 | 0.698585 |
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Target: 5'- cGAGCUCUcCGACCugGUCGAGa-CGGa -3' miRNA: 3'- -CUCGGGAaGCUGGugCAGCUCgaGUU- -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 227097 | 0.72 | 0.628713 |
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Target: 5'- cGGCCgc-CGGCCGgGUCGGGCUCAGa -3' miRNA: 3'- cUCGGgaaGCUGGUgCAGCUCGAGUU- -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 89314 | 0.73 | 0.608655 |
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Target: 5'- -cGCCCUccgucUCGACCAgGUCGgagAGCUCGu -3' miRNA: 3'- cuCGGGA-----AGCUGGUgCAGC---UCGAGUu -5' |
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| 8960 | 3' | -56.6 | NC_002512.2 | + | 210735 | 1.06 | 0.005247 |
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Target: 5'- cGAGCCCUUCGACCACGUCGAGCUCAAg -3' miRNA: 3'- -CUCGGGAAGCUGGUGCAGCUCGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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