Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8975 | 5' | -56 | NC_002512.2 | + | 121525 | 0.68 | 0.89419 |
Target: 5'- aUCCUcGGGGAGcuccgGCGCGCCGggGUCg -3' miRNA: 3'- cGGGA-CCUCUUcua--CGCGCGGUa-CAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 179394 | 0.68 | 0.900563 |
Target: 5'- cGCCUcGGGGA---UGCGCGCCAUc-- -3' miRNA: 3'- -CGGGaCCUCUucuACGCGCGGUAcag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 218092 | 0.67 | 0.906713 |
Target: 5'- uGUCCgUGGGGAAGcgGgcguCGCGCCcgggGUCg -3' miRNA: 3'- -CGGG-ACCUCUUCuaC----GCGCGGua--CAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 32177 | 0.67 | 0.912637 |
Target: 5'- aCCCUGGGGuc---GCGgGCCAUGg- -3' miRNA: 3'- cGGGACCUCuucuaCGCgCGGUACag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 177560 | 0.67 | 0.912637 |
Target: 5'- cGUCUgguucGGGGccgucgccAGGAUGCGCGCCAgcGUCu -3' miRNA: 3'- -CGGGa----CCUC--------UUCUACGCGCGGUa-CAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 60845 | 0.67 | 0.918333 |
Target: 5'- cGCCC--GAGGAGAUGCcgGCGCCGc--- -3' miRNA: 3'- -CGGGacCUCUUCUACG--CGCGGUacag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 72028 | 0.67 | 0.923799 |
Target: 5'- cGCCCUGGAuGGGcagcuUGCGCuGCgCGUGcCg -3' miRNA: 3'- -CGGGACCUcUUCu----ACGCG-CG-GUACaG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 82026 | 0.67 | 0.929036 |
Target: 5'- cGCCCgGGucGGGGU-CGCGCCGgcggcgGUCg -3' miRNA: 3'- -CGGGaCCucUUCUAcGCGCGGUa-----CAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 124001 | 0.67 | 0.929036 |
Target: 5'- aGCCCaccgggugGGAGAcGGAcccGCGCGCCugcGUCc -3' miRNA: 3'- -CGGGa-------CCUCU-UCUa--CGCGCGGua-CAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 163692 | 0.67 | 0.929036 |
Target: 5'- cGCCCUGuccGA--AUGCGUGCCG-GUCg -3' miRNA: 3'- -CGGGACcu-CUucUACGCGCGGUaCAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 185427 | 0.67 | 0.929036 |
Target: 5'- cGCCCUGGAGGucuucGAgGC-CGUCcUGUCc -3' miRNA: 3'- -CGGGACCUCUu----CUaCGcGCGGuACAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 59404 | 0.66 | 0.932565 |
Target: 5'- gGCgCUGGAGAucGAcacgcgcugcaaccUGUGCGCCAUcGUg -3' miRNA: 3'- -CGgGACCUCUu-CU--------------ACGCGCGGUA-CAg -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 220982 | 0.66 | 0.934043 |
Target: 5'- cGCCCUGGccaGGAGGGUcgaGUGCcCCAacggcuucUGUCa -3' miRNA: 3'- -CGGGACC---UCUUCUA---CGCGcGGU--------ACAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 102452 | 0.66 | 0.937884 |
Target: 5'- aCCCgcauggcgucgcGGAGGAGG-GCGCGCCc-GUCg -3' miRNA: 3'- cGGGa-----------CCUCUUCUaCGCGCGGuaCAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 119276 | 0.66 | 0.938821 |
Target: 5'- gGCCCUGGAGGacagcAGcAUGC-UGCUgaugaaGUGUCa -3' miRNA: 3'- -CGGGACCUCU-----UC-UACGcGCGG------UACAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 137059 | 0.66 | 0.938821 |
Target: 5'- gGCCagGGAGGAGgcGcCGCGCCcgGg- -3' miRNA: 3'- -CGGgaCCUCUUCuaC-GCGCGGuaCag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 90102 | 0.66 | 0.938821 |
Target: 5'- cGgCCUGGAGg----GUGCGCCGgacgaUGUCg -3' miRNA: 3'- -CgGGACCUCuucuaCGCGCGGU-----ACAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 32841 | 0.66 | 0.938821 |
Target: 5'- uGCCCaGGcugcAGGAGGUGacgGUGCCGUGg- -3' miRNA: 3'- -CGGGaCC----UCUUCUACg--CGCGGUACag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 76349 | 0.66 | 0.943371 |
Target: 5'- uCCC-GGAGAAGAUGUcgagcgacauGCaGCCGUGa- -3' miRNA: 3'- cGGGaCCUCUUCUACG----------CG-CGGUACag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 123638 | 0.66 | 0.943371 |
Target: 5'- gGUCCcGG-GGAGA-GCGCGCCGcgGUUc -3' miRNA: 3'- -CGGGaCCuCUUCUaCGCGCGGUa-CAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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