Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8988 | 3' | -56.5 | NC_002512.2 | + | 29253 | 0.66 | 0.959553 |
Target: 5'- -cGUC-CUCCGA--CGGGGaCGAgGAGg -3' miRNA: 3'- gaCAGaGAGGCUagGCCCC-GUUgCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 191443 | 0.66 | 0.959553 |
Target: 5'- aUGUUcaCUCCGc-CCGGGGCGuUGAGg -3' miRNA: 3'- gACAGa-GAGGCuaGGCCCCGUuGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 153338 | 0.66 | 0.959553 |
Target: 5'- gCUGgg---CCGGUCCGGGuCGGCGGGu -3' miRNA: 3'- -GACagagaGGCUAGGCCCcGUUGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 43065 | 0.66 | 0.955971 |
Target: 5'- -gGcCUCgccgaggcaCCGGUcCCGGGGCGcgGCGAGc -3' miRNA: 3'- gaCaGAGa--------GGCUA-GGCCCCGU--UGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 130482 | 0.66 | 0.952181 |
Target: 5'- -gGUCggggC-CCGAUCCGucgcagccGGGCuGCGAGg -3' miRNA: 3'- gaCAGa---GaGGCUAGGC--------CCCGuUGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 104283 | 0.66 | 0.948177 |
Target: 5'- -cGUCUUUCuCGcGUCCGGcGGCGG-GAGg -3' miRNA: 3'- gaCAGAGAG-GC-UAGGCC-CCGUUgCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 34767 | 0.66 | 0.948177 |
Target: 5'- -cGcCUCUCCc--CCGGGuCAACGAGg -3' miRNA: 3'- gaCaGAGAGGcuaGGCCCcGUUGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 91885 | 0.66 | 0.948177 |
Target: 5'- -cGUCugaaaguggaUCUCCGucuucUCCuGGGGCGGCGGc -3' miRNA: 3'- gaCAG----------AGAGGCu----AGG-CCCCGUUGCUc -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 202589 | 0.66 | 0.947765 |
Target: 5'- -gGUCUCUCCGAgagcgugacuguuUUCGaGGGCAgaGCGc- -3' miRNA: 3'- gaCAGAGAGGCU-------------AGGC-CCCGU--UGCuc -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 154772 | 0.66 | 0.939519 |
Target: 5'- -cGUCUCcCCGuGUCCGccaGGCGGCGAc -3' miRNA: 3'- gaCAGAGaGGC-UAGGCc--CCGUUGCUc -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 214574 | 0.67 | 0.934861 |
Target: 5'- -cGUCccCUCCcg-CCuGGGCGACGAGa -3' miRNA: 3'- gaCAGa-GAGGcuaGGcCCCGUUGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 67767 | 0.67 | 0.93486 |
Target: 5'- -gGUCgUCUCCGGcgCCGGGGUcguCGGu -3' miRNA: 3'- gaCAG-AGAGGCUa-GGCCCCGuu-GCUc -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 133844 | 0.67 | 0.931959 |
Target: 5'- -gGcCUCUCCGGaggacaccgaggacgUCCuGGGGCGGCucGAGg -3' miRNA: 3'- gaCaGAGAGGCU---------------AGG-CCCCGUUG--CUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 200503 | 0.67 | 0.929981 |
Target: 5'- -cGUC-CUCCGA-CCGGGGCu-CGu- -3' miRNA: 3'- gaCAGaGAGGCUaGGCCCCGuuGCuc -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 131081 | 0.67 | 0.929981 |
Target: 5'- -cGUCgCUCCGGccgCCGGGGUaccugGACGuGg -3' miRNA: 3'- gaCAGaGAGGCUa--GGCCCCG-----UUGCuC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 109470 | 0.67 | 0.92488 |
Target: 5'- ---cCUCUUCGGUCCGGGugaGCAGggUGAGg -3' miRNA: 3'- gacaGAGAGGCUAGGCCC---CGUU--GCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 5493 | 0.67 | 0.92488 |
Target: 5'- gUGgcgCgcgUCCGcgCCGGGGUcGCGAGg -3' miRNA: 3'- gACa--Gag-AGGCuaGGCCCCGuUGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 210476 | 0.67 | 0.92488 |
Target: 5'- uCUGUCUCUCgaggagcaGAUCCGcGaGGCGagggcccgacGCGAGc -3' miRNA: 3'- -GACAGAGAGg-------CUAGGC-C-CCGU----------UGCUC- -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 188557 | 0.67 | 0.919557 |
Target: 5'- -cGUCggaUCCGGUCguguuCGGGGaCAACGAa -3' miRNA: 3'- gaCAGag-AGGCUAG-----GCCCC-GUUGCUc -5' |
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8988 | 3' | -56.5 | NC_002512.2 | + | 141890 | 0.67 | 0.919557 |
Target: 5'- -gGUCuauauaaaUCUCCGcgUCGGGGCGccgcggccccgACGGGg -3' miRNA: 3'- gaCAG--------AGAGGCuaGGCCCCGU-----------UGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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