Results 1 - 20 of 56 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9005 | 3' | -59.4 | NC_002512.2 | + | 15290 | 0.66 | 0.880181 |
Target: 5'- -gUCGGGcugcaggaagagcGUCUCgucgCCCAGGCGGg -3' miRNA: 3'- cgAGCCC-------------UAGAGgcuaGGGUCCGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 122175 | 0.66 | 0.874083 |
Target: 5'- --aCGG--UCUCgGAUCCCAacacGGCGGUg -3' miRNA: 3'- cgaGCCcuAGAGgCUAGGGU----CCGCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 159360 | 0.66 | 0.867828 |
Target: 5'- gGCgUCGGGGUCggggcgucccauucgCgGGUCCCgcggcgucgGGGCGGa -3' miRNA: 3'- -CG-AGCCCUAGa--------------GgCUAGGG---------UCCGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 12413 | 0.66 | 0.867123 |
Target: 5'- cGC-CGGGAUCgcgagcucgCCGcgCgCgAGGCGGg -3' miRNA: 3'- -CGaGCCCUAGa--------GGCuaG-GgUCCGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 96301 | 0.66 | 0.867123 |
Target: 5'- gGC-CGGcccgCUCgGGUCCCcGGCGGUc -3' miRNA: 3'- -CGaGCCcua-GAGgCUAGGGuCCGCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 206129 | 0.66 | 0.867123 |
Target: 5'- gGCgcagCGGGGcgUCCGGUCgCCAGaCGGUc -3' miRNA: 3'- -CGa---GCCCUagAGGCUAG-GGUCcGCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 108445 | 0.66 | 0.867123 |
Target: 5'- gGC-CGGGGggUCCcucCCCGGGCGGc -3' miRNA: 3'- -CGaGCCCUagAGGcuaGGGUCCGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 80855 | 0.66 | 0.859975 |
Target: 5'- cCUCGGGAcgCggggCuCGGUgUCGGGCGGUc -3' miRNA: 3'- cGAGCCCUa-Ga---G-GCUAgGGUCCGCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 27627 | 0.66 | 0.85925 |
Target: 5'- aCUUGGGAUCUCUucgaaaaGAUCCacgaaGCGGUa -3' miRNA: 3'- cGAGCCCUAGAGG-------CUAGGguc--CGCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 8675 | 0.66 | 0.852643 |
Target: 5'- cGUUCGGGAccucUCUCCGuacguacucggGUCCCguccacuccgAGGuCGGUc -3' miRNA: 3'- -CGAGCCCU----AGAGGC-----------UAGGG----------UCC-GCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 224379 | 0.66 | 0.852643 |
Target: 5'- gGUcCGGGAcCUCgCGAcCCCGGcGCGGa -3' miRNA: 3'- -CGaGCCCUaGAG-GCUaGGGUC-CGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 90500 | 0.66 | 0.852643 |
Target: 5'- --aCGGGAUCggggCCGAggCCGgcGGCGGa -3' miRNA: 3'- cgaGCCCUAGa---GGCUagGGU--CCGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 37625 | 0.66 | 0.852643 |
Target: 5'- uGCcgUGGGAUCUCCGAgaCCCGaacguaGCGGc -3' miRNA: 3'- -CGa-GCCCUAGAGGCUa-GGGUc-----CGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 126968 | 0.67 | 0.845132 |
Target: 5'- cGCUCccaguaGGGGUCcgcggUCCucUCCCAGcGCGGg -3' miRNA: 3'- -CGAG------CCCUAG-----AGGcuAGGGUC-CGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 107006 | 0.67 | 0.845132 |
Target: 5'- gGC-CGGGGUCggCCGAgagCCGGGCGc- -3' miRNA: 3'- -CGaGCCCUAGa-GGCUag-GGUCCGCca -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 131680 | 0.67 | 0.837448 |
Target: 5'- uGCUCGGGAaggUCcCCGAgaacgccaUCCCucGGaCGGUc -3' miRNA: 3'- -CGAGCCCU---AGaGGCU--------AGGGu-CC-GCCA- -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 6675 | 0.67 | 0.83667 |
Target: 5'- gGC-CGGGAcgcgCUCCGGgacgccgUCCCcgucGGGCGGc -3' miRNA: 3'- -CGaGCCCUa---GAGGCU-------AGGG----UCCGCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 225838 | 0.67 | 0.829597 |
Target: 5'- cGCggGGGAUCgagCCGcuccuGUCCCGGGUGu- -3' miRNA: 3'- -CGagCCCUAGa--GGC-----UAGGGUCCGCca -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 69126 | 0.67 | 0.821586 |
Target: 5'- cGCUcCGGGAcCUCCGugCCCGGGgcCGGc -3' miRNA: 3'- -CGA-GCCCUaGAGGCuaGGGUCC--GCCa -5' |
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9005 | 3' | -59.4 | NC_002512.2 | + | 183222 | 0.67 | 0.820776 |
Target: 5'- --aCGGGAUCgaccCCGAggaccccUUCCAGGUGGc -3' miRNA: 3'- cgaGCCCUAGa---GGCU-------AGGGUCCGCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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