Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9035 | 3' | -59.9 | NC_002512.2 | + | 228388 | 0.66 | 0.846484 |
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Target: 5'- gGUGUccgGCGUGaUCUguccgggggaCGGGGCGCuGGgGGg -3' miRNA: 3'- gCACG---UGCAC-AGA----------GCCCCGCG-CCaCC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 91584 | 0.66 | 0.846484 |
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Target: 5'- uCGgGCGCGUGUUUCGGGaG-GaaGUGGa -3' miRNA: 3'- -GCaCGUGCACAGAGCCC-CgCgcCACC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 210261 | 0.66 | 0.853873 |
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Target: 5'- gCGUGCGCcgg-CUCGGGGgaGagggaGGUGGg -3' miRNA: 3'- -GCACGUGcacaGAGCCCCg-Cg----CCACC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 104782 | 0.66 | 0.853873 |
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Target: 5'- aCG-GCGCGUGg--CGGGGUccgcgcGCGGcGGg -3' miRNA: 3'- -GCaCGUGCACagaGCCCCG------CGCCaCC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 191028 | 0.66 | 0.861087 |
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Target: 5'- gGUGCcuGCGaGg--CGGGGCGCGGcgcacgUGGa -3' miRNA: 3'- gCACG--UGCaCagaGCCCCGCGCC------ACC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 43076 | 0.66 | 0.861087 |
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Target: 5'- --gGCAcCG-GUCcCGGGGCGCGGcGa -3' miRNA: 3'- gcaCGU-GCaCAGaGCCCCGCGCCaCc -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 82353 | 0.66 | 0.86812 |
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Target: 5'- gGUGgGCGUG-Cg-GGGGCGgCGGaGGg -3' miRNA: 3'- gCACgUGCACaGagCCCCGC-GCCaCC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 90934 | 0.66 | 0.86812 |
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Target: 5'- aCGgGCGCGa--C-CGGGGCGUGcGUGGg -3' miRNA: 3'- -GCaCGUGCacaGaGCCCCGCGC-CACC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 154893 | 0.66 | 0.881628 |
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Target: 5'- --cGCGCGUGUg-CGGGGaggguGCuuGGUGGg -3' miRNA: 3'- gcaCGUGCACAgaGCCCCg----CG--CCACC- -5' |
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| 9035 | 3' | -59.9 | NC_002512.2 | + | 5891 | 0.66 | 0.881628 |
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Target: 5'- uCGUcGCuCGUGgacggCGGGGCGCGcGgGGg -3' miRNA: 3'- -GCA-CGuGCACaga--GCCCCGCGC-CaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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