Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9350 | 5' | -59.3 | NC_002512.2 | + | 105666 | 0.66 | 0.888529 |
Target: 5'- aGCCGcagcaGaCGGCGCGGUaCUCG-CGgugCCUc -3' miRNA: 3'- -CGGCa----C-GUCGCGCCA-GAGCuGCa--GGA- -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 117966 | 0.66 | 0.888529 |
Target: 5'- aGCUGUuccgGCAcccgaacaacccGCGCGGccgCUCGuCGUCCa -3' miRNA: 3'- -CGGCA----CGU------------CGCGCCa--GAGCuGCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 132735 | 0.66 | 0.888529 |
Target: 5'- uGCCGcaggcGCAGCGCGG---CGuCGUCCc -3' miRNA: 3'- -CGGCa----CGUCGCGCCagaGCuGCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 180444 | 0.66 | 0.884636 |
Target: 5'- cCCGgGCGGCGCccgucauuucucgacGG-CUCGGCGUCg- -3' miRNA: 3'- cGGCaCGUCGCG---------------CCaGAGCUGCAGga -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 33081 | 0.66 | 0.881336 |
Target: 5'- aCCG-GCGcGCGCGGUCaUCGGgaccauaucucgcUGUCCg -3' miRNA: 3'- cGGCaCGU-CGCGCCAG-AGCU-------------GCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 91876 | 0.66 | 0.875273 |
Target: 5'- cGCCGUcCGGCGCGGg--CGGCGg--- -3' miRNA: 3'- -CGGCAcGUCGCGCCagaGCUGCagga -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 172144 | 0.66 | 0.875273 |
Target: 5'- aGCgCGacggGCAGCG-GGUCUacuGCGUCCUc -3' miRNA: 3'- -CG-GCa---CGUCGCgCCAGAgc-UGCAGGA- -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 218223 | 0.66 | 0.871143 |
Target: 5'- gGCCGcgGCGGgcCGCGGcgCcgauauauguccgggUCGGCGUCCUc -3' miRNA: 3'- -CGGCa-CGUC--GCGCCa-G---------------AGCUGCAGGA- -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 157166 | 0.66 | 0.868352 |
Target: 5'- gGCCGgGCGGCGUGGgcggCggcgCGACGaUCg- -3' miRNA: 3'- -CGGCaCGUCGCGCCa---Ga---GCUGC-AGga -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 171861 | 0.66 | 0.861241 |
Target: 5'- gGCCGggaggggGCGGaaCGCcGUcCUCGGCGUCCc -3' miRNA: 3'- -CGGCa------CGUC--GCGcCA-GAGCUGCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 207771 | 0.66 | 0.861241 |
Target: 5'- cGCCGUGguGguUGCGGga-CGGCGUCg- -3' miRNA: 3'- -CGGCACguC--GCGCCagaGCUGCAGga -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 168745 | 0.66 | 0.859796 |
Target: 5'- gGCCGUGCAgGCGCugcggaagaccuGGUCguugccguacggCGACagGUCCa -3' miRNA: 3'- -CGGCACGU-CGCG------------CCAGa-----------GCUG--CAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 199877 | 0.66 | 0.853946 |
Target: 5'- uCUGUGCGGCGgcucgcccUGGUCUCGAgacCGcCCg -3' miRNA: 3'- cGGCACGUCGC--------GCCAGAGCU---GCaGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 173917 | 0.67 | 0.846471 |
Target: 5'- gGUCGUGUGGCGUcccguGGUCUCuuuCGUCUc -3' miRNA: 3'- -CGGCACGUCGCG-----CCAGAGcu-GCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 150654 | 0.67 | 0.846471 |
Target: 5'- cGCUGcGCAGCuCGaGcCUCGAgGUCCg -3' miRNA: 3'- -CGGCaCGUCGcGC-CaGAGCUgCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 191590 | 0.67 | 0.846471 |
Target: 5'- aGCC-UGUucggucGCGCGGUCgaguccUCGGCGUCUUc -3' miRNA: 3'- -CGGcACGu-----CGCGCCAG------AGCUGCAGGA- -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 225253 | 0.67 | 0.841902 |
Target: 5'- cCCGUGCuccuucuccgccaggAGgGCGucCUCGGCGUCCg -3' miRNA: 3'- cGGCACG---------------UCgCGCcaGAGCUGCAGGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 95571 | 0.67 | 0.838822 |
Target: 5'- uCCGU-CGGCGcCGGcCccggCGACGUCCUc -3' miRNA: 3'- cGGCAcGUCGC-GCCaGa---GCUGCAGGA- -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 131300 | 0.67 | 0.838822 |
Target: 5'- -aCGUGCAGCGCGGa--CGACccgCCg -3' miRNA: 3'- cgGCACGUCGCGCCagaGCUGca-GGa -5' |
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9350 | 5' | -59.3 | NC_002512.2 | + | 131205 | 0.67 | 0.831006 |
Target: 5'- aGUCGUGC-GCGgccaGGcgCUCGACGUCg- -3' miRNA: 3'- -CGGCACGuCGCg---CCa-GAGCUGCAGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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