Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9373 | 5' | -54.8 | NC_002512.2 | + | 134693 | 0.66 | 0.974617 |
Target: 5'- gCGaCCGCGgacguccucucgcgGGCGCUCUCC--GCCu- -3' miRNA: 3'- -GC-GGCGCaa------------CUGCGAGAGGuuUGGuu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 201823 | 0.66 | 0.97436 |
Target: 5'- cCGCCGCGcccgGGgGCUCcuUCCGcGGCCGg -3' miRNA: 3'- -GCGGCGCaa--CUgCGAG--AGGU-UUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 116137 | 0.66 | 0.97436 |
Target: 5'- aCGCCGCGUaucGugGUcggcaacaUCcCCGAGCCGc -3' miRNA: 3'- -GCGGCGCAa--CugCG--------AGaGGUUUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 226482 | 0.66 | 0.97436 |
Target: 5'- gGCCGgGgcccgGGCGCUCcCCucGCCGc -3' miRNA: 3'- gCGGCgCaa---CUGCGAGaGGuuUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 180563 | 0.66 | 0.97436 |
Target: 5'- uGCgGCGggcuccgGugGCUCUCUAucagGGCCGu -3' miRNA: 3'- gCGgCGCaa-----CugCGAGAGGU----UUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 144304 | 0.66 | 0.971677 |
Target: 5'- --aCGCGgacGCGCUCUCCcGGCCGc -3' miRNA: 3'- gcgGCGCaacUGCGAGAGGuUUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 150164 | 0.66 | 0.971677 |
Target: 5'- aGCC-CGUccuCGCUCUCCcuGCCGGg -3' miRNA: 3'- gCGGcGCAacuGCGAGAGGuuUGGUU- -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 73342 | 0.66 | 0.971677 |
Target: 5'- aGCCGUc---ACGCgCUCCGAACCGg -3' miRNA: 3'- gCGGCGcaacUGCGaGAGGUUUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 76528 | 0.66 | 0.968798 |
Target: 5'- uGCCG-GcUGACGUUCugaugaaggaUCCGAACCGGa -3' miRNA: 3'- gCGGCgCaACUGCGAG----------AGGUUUGGUU- -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 76598 | 0.66 | 0.962427 |
Target: 5'- aCGCCgGCGUcgGACGuCUgUCCGGggccGCCAu -3' miRNA: 3'- -GCGG-CGCAa-CUGC-GAgAGGUU----UGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 176405 | 0.66 | 0.962086 |
Target: 5'- gCGCCGCGUcGugGCaccUgUCCAGcagggugGCCAu -3' miRNA: 3'- -GCGGCGCAaCugCG---AgAGGUU-------UGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 171056 | 0.66 | 0.961399 |
Target: 5'- aCGCCGcCGUccuucacgagaagaUGAgGCUCUUCAcGGCCGu -3' miRNA: 3'- -GCGGC-GCA--------------ACUgCGAGAGGU-UUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 222330 | 0.67 | 0.955204 |
Target: 5'- gGCUGCGgcGGCGC-CUCCAGGagGAg -3' miRNA: 3'- gCGGCGCaaCUGCGaGAGGUUUggUU- -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 101905 | 0.67 | 0.951261 |
Target: 5'- aCGCCGaCGggGGCggcguGUUCUCCAugcgggGACCGAc -3' miRNA: 3'- -GCGGC-GCaaCUG-----CGAGAGGU------UUGGUU- -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 141608 | 0.67 | 0.951261 |
Target: 5'- aGCCGCGUcccgacacgccUGcCGCcaccUCUCCAAcaccGCCGAc -3' miRNA: 3'- gCGGCGCA-----------ACuGCG----AGAGGUU----UGGUU- -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 198365 | 0.67 | 0.950854 |
Target: 5'- cCGCCGUGUccGugGC-CUCCGugaucaggcucugGACCAu -3' miRNA: 3'- -GCGGCGCAa-CugCGaGAGGU-------------UUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 214953 | 0.67 | 0.940872 |
Target: 5'- gGCCGCG--GACGCacgggguccggaUCCAGACCGAc -3' miRNA: 3'- gCGGCGCaaCUGCGag----------AGGUUUGGUU- -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 184377 | 0.67 | 0.938067 |
Target: 5'- cCGCCGCG--GGCGCUUUCgGcguGGCCGc -3' miRNA: 3'- -GCGGCGCaaCUGCGAGAGgU---UUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 221778 | 0.67 | 0.938067 |
Target: 5'- cCGUCGUGUccUGGCGCcugCUCUAcGCCGg -3' miRNA: 3'- -GCGGCGCA--ACUGCGa--GAGGUuUGGUu -5' |
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9373 | 5' | -54.8 | NC_002512.2 | + | 207660 | 0.67 | 0.937592 |
Target: 5'- gGCCGUGgUGGCgucccggGCUCUCCcgccGGCCGAa -3' miRNA: 3'- gCGGCGCaACUG-------CGAGAGGu---UUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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