Results 1 - 20 of 81 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9593 | 3' | -50.5 | NC_002577.1 | + | 132472 | 0.66 | 0.993416 |
Target: 5'- --aGGggGGGAugcGGCGcuguagucGCCGGAu- -3' miRNA: 3'- cuaCCuuCCCUuu-CCGUu-------UGGCCUuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 11045 | 0.66 | 0.993416 |
Target: 5'- cGUGGGAGGGggGcGGCGuuucuagcccccGGCgCGGGc- -3' miRNA: 3'- cUACCUUCCCuuU-CCGU------------UUG-GCCUuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 125119 | 0.66 | 0.993416 |
Target: 5'- gGcgGGggGGGggGGGgGGugUGuGAGu -3' miRNA: 3'- -CuaCCuuCCCuuUCCgUUugGC-CUUu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 1142 | 0.66 | 0.993416 |
Target: 5'- --aGGggGGGAugcGGCGcuguagucGCCGGAu- -3' miRNA: 3'- cuaCCuuCCCUuu-CCGUu-------UGGCCUuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 8495 | 0.66 | 0.993416 |
Target: 5'- gGcgGGggGGGggGGGgGGugUGuGAGu -3' miRNA: 3'- -CuaCCuuCCCuuUCCgUUugGC-CUUu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 122568 | 0.66 | 0.993416 |
Target: 5'- cGUGGGAGGGggGcGGCGuuucuagcccccGGCgCGGGc- -3' miRNA: 3'- cUACCUUCCCuuU-CCGU------------UUG-GCCUuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 19277 | 0.66 | 0.992388 |
Target: 5'- aGAUGGGgcGGGGAAucuauaaaauAGGCAAgguccGCgCGGAu- -3' miRNA: 3'- -CUACCU--UCCCUU----------UCCGUU-----UG-GCCUuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 136702 | 0.66 | 0.992388 |
Target: 5'- -uUGGgcGGGAAAGGC---CCGGc-- -3' miRNA: 3'- cuACCuuCCCUUUCCGuuuGGCCuuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 160636 | 0.66 | 0.992388 |
Target: 5'- -uUGGgcGGGAAAGGC---CCGGc-- -3' miRNA: 3'- cuACCuuCCCUUUCCGuuuGGCCuuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 6081 | 0.66 | 0.989954 |
Target: 5'- --gGGGAGGGggGGGgAAGggaGGAAGa -3' miRNA: 3'- cuaCCUUCCCuuUCCgUUUgg-CCUUU- -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 127533 | 0.66 | 0.989954 |
Target: 5'- --gGGGAGGGggGGGgAAGggaGGAAGa -3' miRNA: 3'- cuaCCUUCCCuuUCCgUUUgg-CCUUU- -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 155313 | 0.66 | 0.989954 |
Target: 5'- uGggGGggGGGggGGGUGGugguGgUGGAGAc -3' miRNA: 3'- -CuaCCuuCCCuuUCCGUU----UgGCCUUU- -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 7582 | 0.67 | 0.985024 |
Target: 5'- gGggGGggGGGggGGGgGAuccgaaccgugcgGCgGGAAGg -3' miRNA: 3'- -CuaCCuuCCCuuUCCgUU-------------UGgCCUUU- -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 126032 | 0.67 | 0.985024 |
Target: 5'- gGggGGggGGGggGGGgGAuccgaaccgugcgGCgGGAAGg -3' miRNA: 3'- -CuaCCuuCCCuuUCCgUU-------------UGgCCUUU- -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 162973 | 0.67 | 0.984081 |
Target: 5'- gGggGGggGGGggGGGCGcgacuccaagcguuaGACCGc--- -3' miRNA: 3'- -CuaCCuuCCCuuUCCGU---------------UUGGCcuuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 134354 | 0.67 | 0.982686 |
Target: 5'- gGggGGggGGGggGGGCGcgacucaagcguuaGACCGc--- -3' miRNA: 3'- -CuaCCuuCCCuuUCCGU--------------UUGGCcuuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 137244 | 0.67 | 0.9812 |
Target: 5'- uGUGGGuc-GAGGGGCGAACCGGu-- -3' miRNA: 3'- cUACCUuccCUUUCCGUUUGGCCuuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 160094 | 0.67 | 0.9812 |
Target: 5'- uGUGGGuc-GAGGGGCGAACCGGu-- -3' miRNA: 3'- cUACCUuccCUUUCCGUUUGGCCuuu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 9629 | 0.68 | 0.978916 |
Target: 5'- --cGGggGGGAGGGGaaaAGAcguuugcguuauCCGGAAu -3' miRNA: 3'- cuaCCuuCCCUUUCCg--UUU------------GGCCUUu -5' |
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9593 | 3' | -50.5 | NC_002577.1 | + | 123984 | 0.68 | 0.978916 |
Target: 5'- --cGGggGGGAGGGGaaaAGAcguuugcguuauCCGGAAu -3' miRNA: 3'- cuaCCuuCCCUUUCCg--UUU------------GGCCUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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