Results 1 - 20 of 89 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11415 | 5' | -55.5 | NC_003085.1 | + | 21939 | 0.73 | 0.297625 |
Target: 5'- cCGGCAUCGCcagcuucagcagggGGugGCUCCGuuGGCUCa -3' miRNA: 3'- uGUUGUAGCG--------------CUugCGAGGCu-CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 48158 | 0.69 | 0.529489 |
Target: 5'- cGCGGCGUCGCGcucACGCUugaggauuUCGGcgcgcacgagguGGCCCa -3' miRNA: 3'- -UGUUGUAGCGCu--UGCGA--------GGCU------------CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 14409 | 0.69 | 0.529489 |
Target: 5'- aGC-GCAUCGCcuuggaGAUGCUCCGcgagggcuGGCCCu -3' miRNA: 3'- -UGuUGUAGCGc-----UUGCGAGGCu-------CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 46729 | 0.69 | 0.5189 |
Target: 5'- gGCAGCGccuUCGCGcGCGCUgCCaGccGCCCg -3' miRNA: 3'- -UGUUGU---AGCGCuUGCGA-GG-CucCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 2348 | 0.69 | 0.508397 |
Target: 5'- uGCGACAUC-CGGgagGCGCagUgCGAGGCCg -3' miRNA: 3'- -UGUUGUAGcGCU---UGCG--AgGCUCCGGg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 34449 | 0.69 | 0.507352 |
Target: 5'- gGCGACGUCGgccagcaUGAGCGuCUCUGGGacgcuGCCCu -3' miRNA: 3'- -UGUUGUAGC-------GCUUGC-GAGGCUC-----CGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 28699 | 0.69 | 0.497985 |
Target: 5'- gACAACG-CGCGGA-GCUgCGcgcuGGCCCu -3' miRNA: 3'- -UGUUGUaGCGCUUgCGAgGCu---CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 21758 | 0.69 | 0.497985 |
Target: 5'- uACGACGUgGCGAGCaUUCU--GGCCCa -3' miRNA: 3'- -UGUUGUAgCGCUUGcGAGGcuCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 2270 | 0.69 | 0.487669 |
Target: 5'- gGCcuCGUCGCGGACuucuucgcggGCUCCGGcagcacggcgcuGGCCUg -3' miRNA: 3'- -UGuuGUAGCGCUUG----------CGAGGCU------------CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 14449 | 0.69 | 0.487669 |
Target: 5'- uACGGCuAUCGuCGAGCGCUUCGcGGaauuCCCu -3' miRNA: 3'- -UGUUG-UAGC-GCUUGCGAGGCuCC----GGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 33072 | 0.7 | 0.447471 |
Target: 5'- uGCuGCAUCGCcaGGACGUgcccgCgGAGGCUCa -3' miRNA: 3'- -UGuUGUAGCG--CUUGCGa----GgCUCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 3574 | 0.71 | 0.409181 |
Target: 5'- gGCGGCAagCGCGccuCGCUgCCGAGGCg- -3' miRNA: 3'- -UGUUGUa-GCGCuu-GCGA-GGCUCCGgg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 227 | 0.71 | 0.409181 |
Target: 5'- gACGACGUCauGCGGACugccuguaaGCUCuucauCGAGGCCUa -3' miRNA: 3'- -UGUUGUAG--CGCUUG---------CGAG-----GCUCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 34539 | 0.71 | 0.390818 |
Target: 5'- cACAGCGgcCGCGAGCGCcaCCagccGGCCCg -3' miRNA: 3'- -UGUUGUa-GCGCUUGCGa-GGcu--CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 11001 | 0.71 | 0.379174 |
Target: 5'- cGCGGCAUCGCGAGuugcuggaagaacuCGUcgaccgCCGAgacaGGCCCg -3' miRNA: 3'- -UGUUGUAGCGCUU--------------GCGa-----GGCU----CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 32916 | 0.72 | 0.364302 |
Target: 5'- uCGACGUCGCGAAUGCggcaCGGcgaccGGCUCa -3' miRNA: 3'- uGUUGUAGCGCUUGCGag--GCU-----CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 7777 | 0.72 | 0.364302 |
Target: 5'- cCAuCGUCGCGGcgaGCGCaUUCGcGGCCCa -3' miRNA: 3'- uGUuGUAGCGCU---UGCG-AGGCuCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 34303 | 0.72 | 0.339057 |
Target: 5'- -gAGCGUCGCGGGCaggGCaCCGAGGCguCCa -3' miRNA: 3'- ugUUGUAGCGCUUG---CGaGGCUCCG--GG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 6665 | 0.72 | 0.339057 |
Target: 5'- cCAAC-UCGUGGACGcCUCCcuGAuGGCCCu -3' miRNA: 3'- uGUUGuAGCGCUUGC-GAGG--CU-CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 33823 | 0.74 | 0.244596 |
Target: 5'- aGCAAUGUUGUGGACGCUcuccgcCCGGccaccGGCCCa -3' miRNA: 3'- -UGUUGUAGCGCUUGCGA------GGCU-----CCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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