Results 1 - 20 of 89 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11415 | 5' | -55.5 | NC_003085.1 | + | 227 | 0.71 | 0.409181 |
Target: 5'- gACGACGUCauGCGGACugccuguaaGCUCuucauCGAGGCCUa -3' miRNA: 3'- -UGUUGUAG--CGCUUG---------CGAG-----GCUCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 966 | 0.67 | 0.616395 |
Target: 5'- -gGAgGUCGC-AGCGCUCaaCGAGGCgCu -3' miRNA: 3'- ugUUgUAGCGcUUGCGAG--GCUCCGgG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 1861 | 0.66 | 0.693249 |
Target: 5'- -aAACAggCGCGGcuccgucuuGCGCUCaucGGCCCa -3' miRNA: 3'- ugUUGUa-GCGCU---------UGCGAGgcuCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 2270 | 0.69 | 0.487669 |
Target: 5'- gGCcuCGUCGCGGACuucuucgcggGCUCCGGcagcacggcgcuGGCCUg -3' miRNA: 3'- -UGuuGUAGCGCUUG----------CGAGGCU------------CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 2348 | 0.69 | 0.508397 |
Target: 5'- uGCGACAUC-CGGgagGCGCagUgCGAGGCCg -3' miRNA: 3'- -UGUUGUAGcGCU---UGCG--AgGCUCCGGg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 2735 | 0.67 | 0.638435 |
Target: 5'- gGCAGCGcgCGCGAagGCGCugcccaaggacUCCGcuGGCCg -3' miRNA: 3'- -UGUUGUa-GCGCU--UGCG-----------AGGCu-CCGGg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 3574 | 0.71 | 0.409181 |
Target: 5'- gGCGGCAagCGCGccuCGCUgCCGAGGCg- -3' miRNA: 3'- -UGUUGUa-GCGCuu-GCGA-GGCUCCGgg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 3982 | 0.67 | 0.649451 |
Target: 5'- gGCcGCGcUUGCGAGCGCgacaCGGcacaguGGCCCa -3' miRNA: 3'- -UGuUGU-AGCGCUUGCGag--GCU------CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 6040 | 0.69 | 0.487669 |
Target: 5'- cACGACGaCGCG-ACGCUCUGuGGGCg- -3' miRNA: 3'- -UGUUGUaGCGCuUGCGAGGC-UCCGgg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 6151 | 0.66 | 0.692163 |
Target: 5'- gGCGACAUgGCGGGacugguggaccugUGCUCCau-GCCCa -3' miRNA: 3'- -UGUUGUAgCGCUU-------------GCGAGGcucCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 6665 | 0.72 | 0.339057 |
Target: 5'- cCAAC-UCGUGGACGcCUCCcuGAuGGCCCu -3' miRNA: 3'- uGUUGuAGCGCUUGC-GAGG--CU-CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 7319 | 0.68 | 0.583462 |
Target: 5'- gACGGcCAUCGCGGAgGCggCUGAGGaCgCg -3' miRNA: 3'- -UGUU-GUAGCGCUUgCGa-GGCUCC-GgG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 7777 | 0.72 | 0.364302 |
Target: 5'- cCAuCGUCGCGGcgaGCGCaUUCGcGGCCCa -3' miRNA: 3'- uGUuGUAGCGCU---UGCG-AGGCuCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 7938 | 0.68 | 0.550893 |
Target: 5'- cCAACucGUUGCGGauGCGUUCCauGGCCCu -3' miRNA: 3'- uGUUG--UAGCGCU--UGCGAGGcuCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 8492 | 0.7 | 0.457351 |
Target: 5'- uGCcGC-UUGCGAGCGCUggCGGGGCCa -3' miRNA: 3'- -UGuUGuAGCGCUUGCGAg-GCUCCGGg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 8941 | 0.66 | 0.670329 |
Target: 5'- uGCAGCG-CGUGGccaaccuGCGCUgggccaagggCCGcGGCCCg -3' miRNA: 3'- -UGUUGUaGCGCU-------UGCGA----------GGCuCCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 10357 | 0.66 | 0.671424 |
Target: 5'- -aGGCAcCGCGGACGCUCCcucaacCCCg -3' miRNA: 3'- ugUUGUaGCGCUUGCGAGGcucc--GGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 10464 | 0.66 | 0.693249 |
Target: 5'- cGCGACGUaGCGGGCucgaauCUgCGGGGCCg -3' miRNA: 3'- -UGUUGUAgCGCUUGc-----GAgGCUCCGGg -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 11001 | 0.71 | 0.379174 |
Target: 5'- cGCGGCAUCGCGAGuugcuggaagaacuCGUcgaccgCCGAgacaGGCCCg -3' miRNA: 3'- -UGUUGUAGCGCUU--------------GCGa-----GGCU----CCGGG- -5' |
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11415 | 5' | -55.5 | NC_003085.1 | + | 11532 | 0.66 | 0.671424 |
Target: 5'- cCGGCcgCGCGuuCGUcgagcgCCGGGGCCa -3' miRNA: 3'- uGUUGuaGCGCuuGCGa-----GGCUCCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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