Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11653 | 3' | -55.8 | NC_003102.1 | + | 40878 | 0.68 | 0.815293 |
Target: 5'- cUCGCGUCGucgaacgugaccagGGCGCC--GUCuuUGCGUc -3' miRNA: 3'- -AGCGCAGC--------------UCGCGGuaCAGuuGCGCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 15655 | 0.68 | 0.817887 |
Target: 5'- aUCGCGgCGAGaCGUCGUGUUggaGGCuaGCg -3' miRNA: 3'- -AGCGCaGCUC-GCGGUACAG---UUGcgCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 40322 | 0.68 | 0.817887 |
Target: 5'- cUCGCaaGUCGAacGCGCCGU-UCGGCG-GCa -3' miRNA: 3'- -AGCG--CAGCU--CGCGGUAcAGUUGCgCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 117927 | 0.68 | 0.826421 |
Target: 5'- aUGU-UCGAGU-CCAUGUCGcuaACGCGCa -3' miRNA: 3'- aGCGcAGCUCGcGGUACAGU---UGCGCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 40779 | 0.67 | 0.850936 |
Target: 5'- aUCGCGUUGAGUauGUCuccauUGUCGGCGUc- -3' miRNA: 3'- -AGCGCAGCUCG--CGGu----ACAGUUGCGcg -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 72150 | 0.66 | 0.913212 |
Target: 5'- aUCGCGUCc-GCGaUCAUGUacugaAGCGCGa -3' miRNA: 3'- -AGCGCAGcuCGC-GGUACAg----UUGCGCg -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 128583 | 0.71 | 0.643972 |
Target: 5'- gCGCGUUGAGCGCgCuagacaGACGCGUc -3' miRNA: 3'- aGCGCAGCUCGCG-Guacag-UUGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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