Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11852 | 3' | -53.5 | NC_003266.2 | + | 10341 | 0.66 | 0.728291 |
Target: 5'- aCGaCUGCgCGCGCCucacGGGccAGACCguGCCc -3' miRNA: 3'- -GCaGAUG-GCGCGG----CUU--UUUGGguCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 16074 | 0.66 | 0.717272 |
Target: 5'- gGUC-AUCGCGCCuGAGAucuacGGCCCcGCg -3' miRNA: 3'- gCAGaUGGCGCGG-CUUU-----UUGGGuCGg -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 12977 | 0.66 | 0.717272 |
Target: 5'- gCGcUCgacaugACCGCGCgCaacauGGAGCCCAGCa -3' miRNA: 3'- -GC-AGa-----UGGCGCG-Gcu---UUUUGGGUCGg -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 16947 | 0.66 | 0.717272 |
Target: 5'- cCGUCgucGCCGCacaGCUGAAcu-CCCGcuGCCg -3' miRNA: 3'- -GCAGa--UGGCG---CGGCUUuuuGGGU--CGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 10053 | 0.66 | 0.717271 |
Target: 5'- gCGcCUAUCGCGCCucaaguaccacGAGcucgcgcGGCCCGGUCa -3' miRNA: 3'- -GCaGAUGGCGCGG-----------CUUu------UUGGGUCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 22115 | 0.66 | 0.706162 |
Target: 5'- gGUCUGCCG-GCCcaugguGGGCaCgCAGCCg -3' miRNA: 3'- gCAGAUGGCgCGGcuu---UUUG-G-GUCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 5811 | 0.66 | 0.706162 |
Target: 5'- aGUgUAggccaCGUGaCCGggGGuCCCGGCCg -3' miRNA: 3'- gCAgAUg----GCGC-GGCuuUUuGGGUCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 28215 | 0.66 | 0.706161 |
Target: 5'- uGUUUACCGUG-CGAagGAAGCCguCAGCa -3' miRNA: 3'- gCAGAUGGCGCgGCU--UUUUGG--GUCGg -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 22321 | 0.66 | 0.706161 |
Target: 5'- gGUCUucuucACCGagggaGCC--AAGGCCCAGCa -3' miRNA: 3'- gCAGA-----UGGCg----CGGcuUUUUGGGUCGg -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 13298 | 0.66 | 0.694974 |
Target: 5'- gGUgCUGCCGCaGCgGu---GCCCgaGGCCg -3' miRNA: 3'- gCA-GAUGGCG-CGgCuuuuUGGG--UCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 24040 | 0.66 | 0.683721 |
Target: 5'- gCGUUUGCgGC-CCGAGucucgugcuGCCCAGaCCc -3' miRNA: 3'- -GCAGAUGgCGcGGCUUuu-------UGGGUC-GG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 17138 | 0.66 | 0.683721 |
Target: 5'- ----aACCGCGCCGuAGAAGgCUGGCg -3' miRNA: 3'- gcagaUGGCGCGGC-UUUUUgGGUCGg -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 2150 | 0.66 | 0.683721 |
Target: 5'- aG-CUACUccucCGCCGGAGggUCCAGgCCg -3' miRNA: 3'- gCaGAUGGc---GCGGCUUUuuGGGUC-GG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 6649 | 0.66 | 0.682593 |
Target: 5'- aGUCaUACaacuCGUGCgagggggCGAGGAGCCCGGCUc -3' miRNA: 3'- gCAG-AUG----GCGCG-------GCUUUUUGGGUCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 9289 | 0.67 | 0.676943 |
Target: 5'- gGUCUcGCCGCGCCGGcgucgcaugguCUCGGUg -3' miRNA: 3'- gCAGA-UGGCGCGGCUuuuu-------GGGUCGg -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 26408 | 0.67 | 0.672415 |
Target: 5'- aGUCcuggGCgGCGCCGGc-GGCCaGGCCc -3' miRNA: 3'- gCAGa---UGgCGCGGCUuuUUGGgUCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 15450 | 0.67 | 0.669015 |
Target: 5'- gGUCUGCgCGCGCCcagcaagauguacgGAGGcGCUC-GCCa -3' miRNA: 3'- gCAGAUG-GCGCGG--------------CUUUuUGGGuCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 8527 | 0.67 | 0.661068 |
Target: 5'- --cCUGCCcgcgcgcgGCGCCGAcguGCCCcuGCCa -3' miRNA: 3'- gcaGAUGG--------CGCGGCUuuuUGGGu-CGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 5370 | 0.67 | 0.649693 |
Target: 5'- aG-CU-CCGCGCC-AAAGGCCacgCGGCCg -3' miRNA: 3'- gCaGAuGGCGCGGcUUUUUGG---GUCGG- -5' |
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11852 | 3' | -53.5 | NC_003266.2 | + | 11259 | 0.67 | 0.647416 |
Target: 5'- gGUCUGCuCGUacGCCGugaccagguuGGCCgCGGCCa -3' miRNA: 3'- gCAGAUG-GCG--CGGCuuu-------UUGG-GUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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