Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12110 | 5' | -56.6 | NC_003309.1 | + | 12026 | 0.66 | 0.645953 |
Target: 5'- gCCGcAGUggACGCGAUCGucucGUCgaaagccugcugcccGCGCGCGg -3' miRNA: 3'- aGGC-UCG--UGUGCUAGC----UAG---------------CGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 21994 | 0.66 | 0.641627 |
Target: 5'- cUCCGcGUGCuCGAugagccguugcUCGAgcCGCGCGCGg -3' miRNA: 3'- -AGGCuCGUGuGCU-----------AGCUa-GCGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 35042 | 0.66 | 0.637301 |
Target: 5'- aCCGAGCGCcugugcgacgccgACGAcgugaucgauaugaUCGGUgaGCGCGCc -3' miRNA: 3'- aGGCUCGUG-------------UGCU--------------AGCUAg-CGCGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 10901 | 0.66 | 0.63081 |
Target: 5'- gCCccGC-CGCGGUCGcgGUCGCcGCGCGu -3' miRNA: 3'- aGGcuCGuGUGCUAGC--UAGCG-CGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 5566 | 0.66 | 0.63081 |
Target: 5'- gUCUucGCGCGCGc-CGAUCGCG-GCGg -3' miRNA: 3'- -AGGcuCGUGUGCuaGCUAGCGCgCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 22460 | 0.66 | 0.684716 |
Target: 5'- aUCGuGCGCgGCGAUCGcacgcugccggcGUCGaGCGCGg -3' miRNA: 3'- aGGCuCGUG-UGCUAGC------------UAGCgCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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