Results 1 - 20 of 93 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12326 | 5' | -54.8 | NC_003324.1 | + | 53384 | 0.67 | 0.631216 |
Target: 5'- cGGCGACcgAUAGuucuuucucaagccGCGaCCGGCgGGCGCg -3' miRNA: 3'- -UCGCUGugUAUU--------------UGC-GGUCGgCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 14529 | 0.66 | 0.744063 |
Target: 5'- cGcCGAUGCuUGGACGCCGGCaugGGCcCa -3' miRNA: 3'- uC-GCUGUGuAUUUGCGGUCGg--CCGuG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 30307 | 0.66 | 0.744063 |
Target: 5'- uGGCGuCGCuc-GGCGCguGCUGGCu- -3' miRNA: 3'- -UCGCuGUGuauUUGCGguCGGCCGug -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 2695 | 0.66 | 0.690029 |
Target: 5'- cAGCGGCAaggGUGAGgcucUGCUAGCCGGauuuGCg -3' miRNA: 3'- -UCGCUGUg--UAUUU----GCGGUCGGCCg---UG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 13175 | 0.67 | 0.656829 |
Target: 5'- cAGCGAggcUGCGUcgGCGgCGGUCGGCGg -3' miRNA: 3'- -UCGCU---GUGUAuuUGCgGUCGGCCGUg -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 23934 | 0.66 | 0.744063 |
Target: 5'- uGCucUACA-AGACGCCGGCCGGaaacUACg -3' miRNA: 3'- uCGcuGUGUaUUUGCGGUCGGCC----GUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 35591 | 0.66 | 0.711896 |
Target: 5'- cGGUG-CGCAgaagucGGCGCuggaagCAGCCGGUACg -3' miRNA: 3'- -UCGCuGUGUau----UUGCG------GUCGGCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 10708 | 0.66 | 0.690029 |
Target: 5'- uAGCGACACGgagccACgGCCAGCguugGGCGa -3' miRNA: 3'- -UCGCUGUGUauu--UG-CGGUCGg---CCGUg -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 31699 | 0.67 | 0.634559 |
Target: 5'- cGCGAgugggUGCAUGccAACGCCAGCCGuuAUg -3' miRNA: 3'- uCGCU-----GUGUAU--UUGCGGUCGGCcgUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 25711 | 0.68 | 0.61228 |
Target: 5'- uGCGugAUGUccaaucAGCaGCCGuGCCGGCGCa -3' miRNA: 3'- uCGCugUGUAu-----UUG-CGGU-CGGCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 7745 | 1.09 | 0.000948 |
Target: 5'- cAGCGACACAUAAACGCCAGCCGGCACu -3' miRNA: 3'- -UCGCUGUGUAUUUGCGGUCGGCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 29872 | 0.66 | 0.73344 |
Target: 5'- cGCGACuguGCGUGugGACGCCgagggauuggAGCCgauccuugGGCGCa -3' miRNA: 3'- uCGCUG---UGUAU--UUGCGG----------UCGG--------CCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 54375 | 0.66 | 0.740888 |
Target: 5'- cAGaCGGCGCA--GACGCaggccuuccagcaaCGGCuCGGCGCu -3' miRNA: 3'- -UC-GCUGUGUauUUGCG--------------GUCG-GCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 30133 | 0.67 | 0.6457 |
Target: 5'- cGCGACcggcgGCGUGAugGgCGGC-GGCAUg -3' miRNA: 3'- uCGCUG-----UGUAUUugCgGUCGgCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 48467 | 0.67 | 0.644587 |
Target: 5'- cAGCcgaGCAgGUAGGCGCCaagggcaacugcgAGCCGGCu- -3' miRNA: 3'- -UCGc--UGUgUAUUUGCGG-------------UCGGCCGug -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 49967 | 0.66 | 0.73344 |
Target: 5'- cGcCGACGCAUGcgaucuuUGCCcGCCGGCuCa -3' miRNA: 3'- uC-GCUGUGUAUuu-----GCGGuCGGCCGuG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 32121 | 0.68 | 0.61228 |
Target: 5'- -uCGGC-CGUGAGcCGCC-GCCGGCAg -3' miRNA: 3'- ucGCUGuGUAUUU-GCGGuCGGCCGUg -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 19097 | 0.68 | 0.601163 |
Target: 5'- uGCGcauCGCGUucGAgGCCAGCCGcGUGCa -3' miRNA: 3'- uCGCu--GUGUAu-UUgCGGUCGGC-CGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 48832 | 0.66 | 0.700997 |
Target: 5'- gAGCGcuuCGC-UGAugGCCugcagccuGCUGGCGCc -3' miRNA: 3'- -UCGCu--GUGuAUUugCGGu-------CGGCCGUG- -5' |
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12326 | 5' | -54.8 | NC_003324.1 | + | 29714 | 0.67 | 0.6457 |
Target: 5'- aGGCGGCgGCGUu-GCGCCAGaagcaGGCAg -3' miRNA: 3'- -UCGCUG-UGUAuuUGCGGUCgg---CCGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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