Results 1 - 20 of 90 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12417 | 3' | -56.6 | NC_003324.1 | + | 15101 | 0.67 | 0.644873 |
Target: 5'- -aUGAUCGACcuGCCGUCGCCuuu-GCCc -3' miRNA: 3'- gaGCUAGCUG--UGGCGGCGGuuccUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 52477 | 0.66 | 0.70877 |
Target: 5'- aUCGGcUCGACcucguagacuuuGCCGCgGCCAGacGGAUg -3' miRNA: 3'- gAGCU-AGCUG------------UGGCGgCGGUU--CCUGg -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 22181 | 0.66 | 0.70877 |
Target: 5'- gCUCGAUCGuC-UgGCgCGCCGAGaACCu -3' miRNA: 3'- -GAGCUAGCuGuGgCG-GCGGUUCcUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 32422 | 0.66 | 0.677005 |
Target: 5'- -cCGggCGAUGCCGCUaggcagGCCAugcAGGAUCc -3' miRNA: 3'- gaGCuaGCUGUGGCGG------CGGU---UCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 31180 | 0.66 | 0.677005 |
Target: 5'- -cCGAgcagcagCG-CAUUGCCG-CAAGGACCg -3' miRNA: 3'- gaGCUa------GCuGUGGCGGCgGUUCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 19393 | 0.66 | 0.70877 |
Target: 5'- gCUCcg-CGAgCGCUacauggcuauGCCGcCCAAGGGCCg -3' miRNA: 3'- -GAGcuaGCU-GUGG----------CGGC-GGUUCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 24632 | 0.66 | 0.697183 |
Target: 5'- -aCGAUcuucCGGCACUGCUcuccaagGCCAuccGGACCc -3' miRNA: 3'- gaGCUA----GCUGUGGCGG-------CGGUu--CCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 25940 | 0.66 | 0.681267 |
Target: 5'- gUCGAUCGcgaucgcuccaccacGCGCCGCauccaGCUuGAGGACg -3' miRNA: 3'- gAGCUAGC---------------UGUGGCGg----CGG-UUCCUGg -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 13416 | 0.66 | 0.655608 |
Target: 5'- uUUGGUcCGGCgcgGCUGCCGUCGAGcACCu -3' miRNA: 3'- gAGCUA-GCUG---UGGCGGCGGUUCcUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 38323 | 0.67 | 0.634128 |
Target: 5'- -gCGAUCGGCAuuCCGgCGCUgaaaGAGGAUUu -3' miRNA: 3'- gaGCUAGCUGU--GGCgGCGG----UUCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 32571 | 0.79 | 0.128812 |
Target: 5'- aUCG-UCGACACCGUgauCGCCGAGGcuGCCg -3' miRNA: 3'- gAGCuAGCUGUGGCG---GCGGUUCC--UGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 23626 | 0.66 | 0.698239 |
Target: 5'- -cCGAgCG-CGcCCGCCGCCAcgcuguGGGGCa -3' miRNA: 3'- gaGCUaGCuGU-GGCGGCGGU------UCCUGg -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 49595 | 0.66 | 0.702459 |
Target: 5'- gCUCGAUaCGGCcgagagugccagaauGCCGCCcucGUUgcGGACCg -3' miRNA: 3'- -GAGCUA-GCUG---------------UGGCGG---CGGuuCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 48268 | 0.66 | 0.666321 |
Target: 5'- uCUCGGagaaccagCGaACGCgGCCGaUAGGGACCa -3' miRNA: 3'- -GAGCUa-------GC-UGUGgCGGCgGUUCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 26060 | 0.66 | 0.655608 |
Target: 5'- aUCGAUCuggucuggGGCGCCGaaggcaccaaCGCCAgcaauggucAGGGCCu -3' miRNA: 3'- gAGCUAG--------CUGUGGCg---------GCGGU---------UCCUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 5540 | 0.66 | 0.698239 |
Target: 5'- -gCGggCGACGCCGCCuGCCu---ACCc -3' miRNA: 3'- gaGCuaGCUGUGGCGG-CGGuuccUGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 15284 | 0.67 | 0.634128 |
Target: 5'- uUCGAgguuUCGuCcCCGCCGUCAcGGGCg -3' miRNA: 3'- gAGCU----AGCuGuGGCGGCGGUuCCUGg -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 6823 | 0.67 | 0.630904 |
Target: 5'- uCUgGAUCGGCGCCGuuGgCCAccuuuaccgaugucGGGAg- -3' miRNA: 3'- -GAgCUAGCUGUGGCggC-GGU--------------UCCUgg -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 49506 | 0.66 | 0.687647 |
Target: 5'- --aGAUCagccaaGCGCCGCUGCCGGGcucGCCg -3' miRNA: 3'- gagCUAGc-----UGUGGCGGCGGUUCc--UGG- -5' |
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12417 | 3' | -56.6 | NC_003324.1 | + | 34719 | 0.66 | 0.677005 |
Target: 5'- gCUCu-UCGACauGCUGgaCGCCGAGGugCg -3' miRNA: 3'- -GAGcuAGCUG--UGGCg-GCGGUUCCugG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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