Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12431 | 3' | -61.6 | NC_003324.1 | + | 8243 | 0.66 | 0.45418 |
Target: 5'- aCGaaGCUGACGCGGCgUUUGcCUGcgaGCGGg -3' miRNA: 3'- -GCggCGGCUGCGUCG-GAGC-GAC---UGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 21931 | 0.66 | 0.45418 |
Target: 5'- gGCCGCCGAgGaGGCC-CgGCUGcGCGa -3' miRNA: 3'- gCGGCGGCUgCgUCGGaG-CGAC-UGCc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 18828 | 0.66 | 0.45418 |
Target: 5'- gGCU-CCGGCGuCAGCaacgaGCUGAUGGa -3' miRNA: 3'- gCGGcGGCUGC-GUCGgag--CGACUGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 6169 | 0.66 | 0.453245 |
Target: 5'- gCGCUGCCcgacgacaugcgaGAgGCAGcCCUUGCUG-CGa -3' miRNA: 3'- -GCGGCGG-------------CUgCGUC-GGAGCGACuGCc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 42868 | 0.66 | 0.444877 |
Target: 5'- gGCUGCCGGC-CGGCC-CGCgagGAUc- -3' miRNA: 3'- gCGGCGGCUGcGUCGGaGCGa--CUGcc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 14682 | 0.66 | 0.444877 |
Target: 5'- gGCCGCC-ACGCuaccGCCUgCGCcGcCGGc -3' miRNA: 3'- gCGGCGGcUGCGu---CGGA-GCGaCuGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 31246 | 0.66 | 0.435682 |
Target: 5'- gCGCCGCCGGcCGCAcuGCgcguuUUUGC-GGCGGc -3' miRNA: 3'- -GCGGCGGCU-GCGU--CG-----GAGCGaCUGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 5057 | 0.66 | 0.435682 |
Target: 5'- cCGCCGCCaaggacccgaACGCGGCCUgauaGCUuaaGCGGc -3' miRNA: 3'- -GCGGCGGc---------UGCGUCGGAg---CGAc--UGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 6303 | 0.66 | 0.435682 |
Target: 5'- gCGCUGCUGcuGCGCcucGGCCUUcaugGCUucGACGGu -3' miRNA: 3'- -GCGGCGGC--UGCG---UCGGAG----CGA--CUGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 20173 | 0.66 | 0.426598 |
Target: 5'- aCGCUGCCG--GCAGCCUCGgUcagagaGGCGc -3' miRNA: 3'- -GCGGCGGCugCGUCGGAGCgA------CUGCc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 16894 | 0.66 | 0.417627 |
Target: 5'- uGCCGCCuuGAUGCuuGCCgucacccUGCUGGcCGGa -3' miRNA: 3'- gCGGCGG--CUGCGu-CGGa------GCGACU-GCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 49971 | 0.66 | 0.417627 |
Target: 5'- aGCgCGCCGACGCAugcgaucuuuGCC-CGCcGGCu- -3' miRNA: 3'- gCG-GCGGCUGCGU----------CGGaGCGaCUGcc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 2760 | 0.66 | 0.408773 |
Target: 5'- uGCCcuuGCCGAaGCGGCCaccgCGCUGgaaGGg -3' miRNA: 3'- gCGG---CGGCUgCGUCGGa---GCGACug-CC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 1743 | 0.66 | 0.407894 |
Target: 5'- cCGCCGUCaGCGuCAGCaggCGCaggccgaguuucgUGACGGg -3' miRNA: 3'- -GCGGCGGcUGC-GUCGga-GCG-------------ACUGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 12427 | 0.67 | 0.400036 |
Target: 5'- uGCUGCCGAgCGCGGCaaUGCcGACa- -3' miRNA: 3'- gCGGCGGCU-GCGUCGgaGCGaCUGcc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 19538 | 0.67 | 0.400036 |
Target: 5'- aGCCGCUucacGCGCAuauaCUCGCcGACGGc -3' miRNA: 3'- gCGGCGGc---UGCGUcg--GAGCGaCUGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 41723 | 0.67 | 0.391419 |
Target: 5'- gCGCCGCCGACGagagcgggcgAGCCgUgGCaaugGugGGc -3' miRNA: 3'- -GCGGCGGCUGCg---------UCGG-AgCGa---CugCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 21086 | 0.67 | 0.391419 |
Target: 5'- gGCCGCCGcggcgcgugcuGCGuCGGCUUCcacgucggGCUGGCGc -3' miRNA: 3'- gCGGCGGC-----------UGC-GUCGGAG--------CGACUGCc -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 2412 | 0.67 | 0.374553 |
Target: 5'- cCGCCaacGCCGAUGaugaAGCCgccgaCGCcGGCGGc -3' miRNA: 3'- -GCGG---CGGCUGCg---UCGGa----GCGaCUGCC- -5' |
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12431 | 3' | -61.6 | NC_003324.1 | + | 36174 | 0.67 | 0.374553 |
Target: 5'- aCGCCGCCGACcugcuGCAGCaua-CUGAUGu -3' miRNA: 3'- -GCGGCGGCUG-----CGUCGgagcGACUGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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