Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12446 | 3' | -53.6 | NC_003324.1 | + | 34420 | 0.68 | 0.668498 |
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Target: 5'- cUCGGCaucGGUGAUGUCGGcCGACggCAc -3' miRNA: 3'- uAGCUG---CCGUUACAGCC-GCUGuaGUu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 39745 | 0.68 | 0.679703 |
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Target: 5'- -aUGGCGGCcuuGGUGUcCGGCGGCAcgugcUCGAu -3' miRNA: 3'- uaGCUGCCG---UUACA-GCCGCUGU-----AGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 7650 | 0.67 | 0.734779 |
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Target: 5'- aGUCGcgcCGGCAGUGcCGGCuGGCGUUu- -3' miRNA: 3'- -UAGCu--GCCGUUACaGCCG-CUGUAGuu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 20803 | 0.66 | 0.745519 |
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Target: 5'- --aGGCGGCGgaccGUGUUGGUGACGcgCAu -3' miRNA: 3'- uagCUGCCGU----UACAGCCGCUGUa-GUu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 43147 | 0.66 | 0.766623 |
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Target: 5'- cUCGACGGCGAgcuUGUUGaGCGAgCGcCAAu -3' miRNA: 3'- uAGCUGCCGUU---ACAGC-CGCU-GUaGUU- -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 1528 | 0.66 | 0.766623 |
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Target: 5'- -cCGGCGGCAGUGgugcgCGGCGcUAUg-- -3' miRNA: 3'- uaGCUGCCGUUACa----GCCGCuGUAguu -5' |
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| 12446 | 3' | -53.6 | NC_003324.1 | + | 26453 | 1.04 | 0.002873 |
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Target: 5'- cAUCGACGGCAAUGUCGGCGACAUCAAu -3' miRNA: 3'- -UAGCUGCCGUUACAGCCGCUGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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