Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12446 | 5' | -56.1 | NC_003324.1 | + | 13165 | 0.7 | 0.47076 |
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Target: 5'- gCgUCGGCGGCGgUCGGCggcggcgGUACCGg-- -3' miRNA: 3'- -GgAGCCGUCGCgAGCUG-------UAUGGCagc -5' |
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| 12446 | 5' | -56.1 | NC_003324.1 | + | 12360 | 0.68 | 0.596195 |
|
Target: 5'- gCUCGGCAGCauuCUCGugGUAgUGaCGa -3' miRNA: 3'- gGAGCCGUCGc--GAGCugUAUgGCaGC- -5' |
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| 12446 | 5' | -56.1 | NC_003324.1 | + | 11009 | 0.71 | 0.442625 |
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Target: 5'- aCCcCGGCGGUGUUgGcauACAUACCGaUCGg -3' miRNA: 3'- -GGaGCCGUCGCGAgC---UGUAUGGC-AGC- -5' |
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| 12446 | 5' | -56.1 | NC_003324.1 | + | 8327 | 0.68 | 0.596195 |
|
Target: 5'- --cCGuCGGCGCUCGuuACGgugACCGUCGg -3' miRNA: 3'- ggaGCcGUCGCGAGC--UGUa--UGGCAGC- -5' |
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| 12446 | 5' | -56.1 | NC_003324.1 | + | 6377 | 0.68 | 0.596195 |
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Target: 5'- ---aGGCuauuGUGCUCGcCAaGCCGUCGg -3' miRNA: 3'- ggagCCGu---CGCGAGCuGUaUGGCAGC- -5' |
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| 12446 | 5' | -56.1 | NC_003324.1 | + | 5126 | 0.66 | 0.692784 |
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Target: 5'- uCCUUGGCGGCGgugucgaUCGAgAUcacGCCGUa- -3' miRNA: 3'- -GGAGCCGUCGCg------AGCUgUA---UGGCAgc -5' |
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| 12446 | 5' | -56.1 | NC_003324.1 | + | 1991 | 0.72 | 0.36181 |
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Target: 5'- --aCGGCuuCGCUCGACGUucucaACCGUCa -3' miRNA: 3'- ggaGCCGucGCGAGCUGUA-----UGGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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