Results 1 - 20 of 79 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12462 | 3' | -56.1 | NC_003324.1 | + | 25606 | 0.66 | 0.694607 |
Target: 5'- --gUCUGUCGGCCGcugcuGCGCcGGcACGGCu -3' miRNA: 3'- guaAGGCGGCUGGC-----UGUGcUC-UGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 43173 | 0.66 | 0.726094 |
Target: 5'- ---cCCGCUGGCgGAUgGCGAGACGa- -3' miRNA: 3'- guaaGGCGGCUGgCUG-UGCUCUGCcg -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 17506 | 0.66 | 0.726094 |
Target: 5'- --cUCCGCgucaGACCGACgaggGCGAcgUGGCa -3' miRNA: 3'- guaAGGCGg---CUGGCUG----UGCUcuGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 47602 | 0.66 | 0.736425 |
Target: 5'- aGUUCCGuCCGAgcuuuaccaCGcCGCGcauGACGGCg -3' miRNA: 3'- gUAAGGC-GGCUg--------GCuGUGCu--CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 54075 | 0.66 | 0.726094 |
Target: 5'- --aUCgGCCGACCGGCuCGAcauccaguuuguGACGaaGCg -3' miRNA: 3'- guaAGgCGGCUGGCUGuGCU------------CUGC--CG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 42259 | 0.66 | 0.726094 |
Target: 5'- gGUUCuCGUugaGACCuacagGGCGCGGGuCGGCa -3' miRNA: 3'- gUAAG-GCGg--CUGG-----CUGUGCUCuGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 23517 | 0.66 | 0.736425 |
Target: 5'- gGUUCCaaccCCGGCaCGAUcggugccccacaGCGuGGCGGCg -3' miRNA: 3'- gUAAGGc---GGCUG-GCUG------------UGCuCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 5965 | 0.66 | 0.736425 |
Target: 5'- ---cCCGCCGAauGGCuaugcagcGCGAGcACGGUg -3' miRNA: 3'- guaaGGCGGCUggCUG--------UGCUC-UGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2418 | 0.66 | 0.736425 |
Target: 5'- ----aCGCCGaugaugaagccGCCGACGCc-GGCGGCu -3' miRNA: 3'- guaagGCGGC-----------UGGCUGUGcuCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2687 | 0.66 | 0.736425 |
Target: 5'- ---gCCGCuuCGGCaagGGCACGAugucGGCGGCg -3' miRNA: 3'- guaaGGCG--GCUGg--CUGUGCU----CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 23924 | 0.66 | 0.726094 |
Target: 5'- ----gCGCCGACaUGGCGuCGAGguucacGCGGCg -3' miRNA: 3'- guaagGCGGCUG-GCUGU-GCUC------UGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2529 | 0.66 | 0.726094 |
Target: 5'- ----aCGCC-ACCGACgaacagGCGAcgGACGGCa -3' miRNA: 3'- guaagGCGGcUGGCUG------UGCU--CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 49101 | 0.66 | 0.725056 |
Target: 5'- cUAUUUCGCUG-CCgguGACGCGAucgccaaGAUGGCg -3' miRNA: 3'- -GUAAGGCGGCuGG---CUGUGCU-------CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 29911 | 0.66 | 0.715674 |
Target: 5'- ---gCUGCUGGCC-AUGCuGGACGGCg -3' miRNA: 3'- guaaGGCGGCUGGcUGUGcUCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 30060 | 0.66 | 0.715674 |
Target: 5'- uCAcgCCGCCGgucgcgacACCGGC-CGcuGCGGCu -3' miRNA: 3'- -GUaaGGCGGC--------UGGCUGuGCucUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 23724 | 0.66 | 0.705174 |
Target: 5'- --gUCCGCCGGCUG-CACGucACGa- -3' miRNA: 3'- guaAGGCGGCUGGCuGUGCucUGCcg -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 11594 | 0.66 | 0.705174 |
Target: 5'- --cUCCuGCCGGa-GACugGugcGACGGCa -3' miRNA: 3'- guaAGG-CGGCUggCUGugCu--CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 52092 | 0.66 | 0.704121 |
Target: 5'- ---cCUGCCGuaaggacGCCGACgauuauuuugACGAGGuCGGCg -3' miRNA: 3'- guaaGGCGGC-------UGGCUG----------UGCUCU-GCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 31021 | 0.66 | 0.704121 |
Target: 5'- ---aUCGCuugguagCGACCGGCGCG-GuCGGCg -3' miRNA: 3'- guaaGGCG-------GCUGGCUGUGCuCuGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 14581 | 0.66 | 0.694607 |
Target: 5'- ---cCUGCCGgcgcaagcgGCCGGCgGCGcAGGCGGUa -3' miRNA: 3'- guaaGGCGGC---------UGGCUG-UGC-UCUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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