Results 1 - 20 of 79 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12462 | 3' | -56.1 | NC_003324.1 | + | 1663 | 0.68 | 0.608835 |
Target: 5'- --cUgCGCCuGCUGACGCu-GACGGCg -3' miRNA: 3'- guaAgGCGGcUGGCUGUGcuCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2418 | 0.66 | 0.736425 |
Target: 5'- ----aCGCCGaugaugaagccGCCGACGCc-GGCGGCu -3' miRNA: 3'- guaagGCGGC-----------UGGCUGUGcuCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2529 | 0.66 | 0.726094 |
Target: 5'- ----aCGCC-ACCGACgaacagGCGAcgGACGGCa -3' miRNA: 3'- guaagGCGGcUGGCUG------UGCU--CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2687 | 0.66 | 0.736425 |
Target: 5'- ---gCCGCuuCGGCaagGGCACGAugucGGCGGCg -3' miRNA: 3'- guaaGGCG--GCUGg--CUGUGCU----CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 2776 | 0.68 | 0.587401 |
Target: 5'- ---gCCGCCG-CCGACAucgugcccuugcCGAaGCGGCc -3' miRNA: 3'- guaaGGCGGCuGGCUGU------------GCUcUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 4210 | 0.66 | 0.715674 |
Target: 5'- ---gUCGCCGGcuuCCGAUuCGAGAuCGGUg -3' miRNA: 3'- guaaGGCGGCU---GGCUGuGCUCU-GCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 5965 | 0.66 | 0.736425 |
Target: 5'- ---cCCGCCGAauGGCuaugcagcGCGAGcACGGUg -3' miRNA: 3'- guaaGGCGGCUggCUG--------UGCUC-UGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 7693 | 0.67 | 0.630346 |
Target: 5'- ---aCCGCCGAccccggaaacaCCGGCGCGAacgaggucaGcACGGCc -3' miRNA: 3'- guaaGGCGGCU-----------GGCUGUGCU---------C-UGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 8416 | 0.67 | 0.673309 |
Target: 5'- ---cCCGCCGACgGucaccguaACGAgcgccGACGGCg -3' miRNA: 3'- guaaGGCGGCUGgCug------UGCU-----CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 8425 | 0.69 | 0.534574 |
Target: 5'- -uUUCCGCCGGCUG-CAgGugcAGGCGGa -3' miRNA: 3'- guAAGGCGGCUGGCuGUgC---UCUGCCg -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 9382 | 0.66 | 0.715674 |
Target: 5'- gGUgCCGCC-ACCGAUgACGAGGucuCGGUa -3' miRNA: 3'- gUAaGGCGGcUGGCUG-UGCUCU---GCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 9647 | 0.7 | 0.463758 |
Target: 5'- ---aCCGCCGgcaacGCCaACGCGcuGGGCGGCg -3' miRNA: 3'- guaaGGCGGC-----UGGcUGUGC--UCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 11594 | 0.66 | 0.705174 |
Target: 5'- --cUCCuGCCGGa-GACugGugcGACGGCa -3' miRNA: 3'- guaAGG-CGGCUggCUGugCu--CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 11841 | 0.68 | 0.619585 |
Target: 5'- --gUCCuGCgCGACCgGGC-CGGGcACGGCa -3' miRNA: 3'- guaAGG-CG-GCUGG-CUGuGCUC-UGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 13088 | 0.69 | 0.524197 |
Target: 5'- ---gCCGCCGACCGcCGcCGAcgcagccucgcuGACGGUu -3' miRNA: 3'- guaaGGCGGCUGGCuGU-GCU------------CUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 13787 | 0.66 | 0.705174 |
Target: 5'- ---gCCGCCGuCCGACGCGAcACu-- -3' miRNA: 3'- guaaGGCGGCuGGCUGUGCUcUGccg -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 14532 | 0.67 | 0.673309 |
Target: 5'- --gUCCGCCGaugcuuggacGCCGGCAUGGGcCcagagccgGGCg -3' miRNA: 3'- guaAGGCGGC----------UGGCUGUGCUCuG--------CCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 14581 | 0.66 | 0.694607 |
Target: 5'- ---cCUGCCGgcgcaagcgGCCGGCgGCGcAGGCGGUa -3' miRNA: 3'- guaaGGCGGC---------UGGCUG-UGC-UCUGCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 15248 | 0.68 | 0.619585 |
Target: 5'- --aUCUGCgGaACCGGCGaugGAGAuCGGCg -3' miRNA: 3'- guaAGGCGgC-UGGCUGUg--CUCU-GCCG- -5' |
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12462 | 3' | -56.1 | NC_003324.1 | + | 15571 | 0.68 | 0.619585 |
Target: 5'- gGUUCagaaGUgGGCCGACACGgcagaugccaAGGCGGg -3' miRNA: 3'- gUAAGg---CGgCUGGCUGUGC----------UCUGCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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