Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12485 | 5' | -57.1 | NC_003324.1 | + | 9654 | 0.67 | 0.525711 |
Target: 5'- -gCAACGCCaACG-CGCUGggcggcguggggcCGGGCGGa -3' miRNA: 3'- aaGUUGCGGgUGUaGCGAC-------------GUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 5429 | 0.67 | 0.526766 |
Target: 5'- -gCGGCGCcgaguucuucuCCGCAUCGaCgcugggucagGCAGGCGGc -3' miRNA: 3'- aaGUUGCG-----------GGUGUAGC-Ga---------CGUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 19384 | 0.67 | 0.526766 |
Target: 5'- aUCGACGCCggcgCGCGUCGCgccgUGCAugaGGuCGGc -3' miRNA: 3'- aAGUUGCGG----GUGUAGCG----ACGU---CC-GCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 53738 | 0.67 | 0.537354 |
Target: 5'- cUCGGCGCuCCAaggUGCcGCAcGGCGGu -3' miRNA: 3'- aAGUUGCG-GGUguaGCGaCGU-CCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 40925 | 0.67 | 0.548015 |
Target: 5'- aUCAGCaaUCAC--CGCUGCgAGGCGGa -3' miRNA: 3'- aAGUUGcgGGUGuaGCGACG-UCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 4882 | 0.67 | 0.548015 |
Target: 5'- gUUCAugGgCCGucaggcuggcguUAUCGCUuucggcuccGCAGGCGGc -3' miRNA: 3'- -AAGUugCgGGU------------GUAGCGA---------CGUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 39460 | 0.67 | 0.548015 |
Target: 5'- cUCGAUGCgCCACAUCGg-GUcGGCGa -3' miRNA: 3'- aAGUUGCG-GGUGUAGCgaCGuCCGCc -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 49706 | 0.67 | 0.548015 |
Target: 5'- -cCGACGgcacuuauCCCGCugccCUGCAGGCGGg -3' miRNA: 3'- aaGUUGC--------GGGUGuagcGACGUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 31133 | 0.67 | 0.548015 |
Target: 5'- gUUCuuGCGCCUgg--CGCUGCAGGCc- -3' miRNA: 3'- -AAGu-UGCGGGuguaGCGACGUCCGcc -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 43814 | 0.67 | 0.557666 |
Target: 5'- uUUCAcagucGCGaCCCugAUCccgauugGCaGCAGGCGGu -3' miRNA: 3'- -AAGU-----UGC-GGGugUAG-------CGaCGUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 29459 | 0.66 | 0.569527 |
Target: 5'- cUCGACGaugacacggCCGCcUgGCUGCGGGCGc -3' miRNA: 3'- aAGUUGCg--------GGUGuAgCGACGUCCGCc -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 9727 | 0.66 | 0.580362 |
Target: 5'- -cCAGCGCgUugG-CGUUGCcGGCGGu -3' miRNA: 3'- aaGUUGCGgGugUaGCGACGuCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 24798 | 0.66 | 0.59124 |
Target: 5'- -cCGGCGUUCuugaggaagGCAUUGUucgGCAGGCGGa -3' miRNA: 3'- aaGUUGCGGG---------UGUAGCGa--CGUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 45767 | 0.66 | 0.59124 |
Target: 5'- aUCAugGCCUGCcaCGCcuuccGCAGaGCGGa -3' miRNA: 3'- aAGUugCGGGUGuaGCGa----CGUC-CGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 50099 | 0.66 | 0.602153 |
Target: 5'- aUCAGCgGCCC-CAgCGCuugccgaucuUGUGGGCGGc -3' miRNA: 3'- aAGUUG-CGGGuGUaGCG----------ACGUCCGCC- -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 48621 | 0.66 | 0.602153 |
Target: 5'- gUCGGCGCCCGCAUCcgGCUccgccGCAacaucaucGGCa- -3' miRNA: 3'- aAGUUGCGGGUGUAG--CGA-----CGU--------CCGcc -5' |
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12485 | 5' | -57.1 | NC_003324.1 | + | 36867 | 0.66 | 0.624045 |
Target: 5'- aUCAGCGCCUuccgcuuggggaACAUCGC-GCAaccaaugucGGCGu -3' miRNA: 3'- aAGUUGCGGG------------UGUAGCGaCGU---------CCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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