Results 1 - 20 of 100 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13951 | 3' | -53.3 | NC_003521.1 | + | 27381 | 0.66 | 0.992117 |
Target: 5'- aUGGaGUUCGCCCgcaCCGGCCuGCGcgccGUCg -3' miRNA: 3'- -ACUgCAAGUGGGag-GGUUGG-CGU----UAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 117251 | 0.66 | 0.992117 |
Target: 5'- cGGCGccgcCGCCaUCCCGccACCGCcGUCg -3' miRNA: 3'- aCUGCaa--GUGGgAGGGU--UGGCGuUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 27837 | 0.66 | 0.992117 |
Target: 5'- gGACGUg--UUCgUCCAGCCGCGGUCu -3' miRNA: 3'- aCUGCAaguGGGaGGGUUGGCGUUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 212082 | 0.66 | 0.992117 |
Target: 5'- aGACGgu--CCC-CCgGACCGCAAa- -3' miRNA: 3'- aCUGCaaguGGGaGGgUUGGCGUUag -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 118902 | 0.66 | 0.991007 |
Target: 5'- aUGAgGUccUCGCCCUCgUCGgcGCCGCGGc- -3' miRNA: 3'- -ACUgCA--AGUGGGAG-GGU--UGGCGUUag -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 111464 | 0.66 | 0.991007 |
Target: 5'- cGGCGccaUCGCCCagCCCAccguCCGCGgcgccGUCg -3' miRNA: 3'- aCUGCa--AGUGGGa-GGGUu---GGCGU-----UAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 49198 | 0.66 | 0.991007 |
Target: 5'- --cCGcUUCuCCCUCCCcGCCGCugauAGUCg -3' miRNA: 3'- acuGC-AAGuGGGAGGGuUGGCG----UUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 58100 | 0.66 | 0.991007 |
Target: 5'- cGGCGUUCGgCgUCaCCGGCCGagcuUCa -3' miRNA: 3'- aCUGCAAGUgGgAG-GGUUGGCguu-AG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 49351 | 0.66 | 0.991007 |
Target: 5'- uUGugGauaUCGCCC-CCCGuCCGaCGGUCc -3' miRNA: 3'- -ACugCa--AGUGGGaGGGUuGGC-GUUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 215934 | 0.66 | 0.99089 |
Target: 5'- cGGCGUucuggucgcgcuuUCGCUgCUCCCG-CCGCGAc- -3' miRNA: 3'- aCUGCA-------------AGUGG-GAGGGUuGGCGUUag -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 17896 | 0.66 | 0.990651 |
Target: 5'- cGGUGUUCucuUCCUCCCAgcggguucguauacGCCGCGAc- -3' miRNA: 3'- aCUGCAAGu--GGGAGGGU--------------UGGCGUUag -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 236378 | 0.66 | 0.990651 |
Target: 5'- cGuCGUUCuuCCUCCUAccgacguaccucuaACCGCGGUg -3' miRNA: 3'- aCuGCAAGugGGAGGGU--------------UGGCGUUAg -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 12238 | 0.66 | 0.989778 |
Target: 5'- cGACGgcagaccUCACUCUCgcugCCgAGCUGCAGUCc -3' miRNA: 3'- aCUGCa------AGUGGGAG----GG-UUGGCGUUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 66601 | 0.66 | 0.989778 |
Target: 5'- cGACGcgC-CCCUgCCCGACCcGCGc-- -3' miRNA: 3'- aCUGCaaGuGGGA-GGGUUGG-CGUuag -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 108534 | 0.66 | 0.989778 |
Target: 5'- cGACGgcgaccuggUCACCCggacCCUGGCCGUAu-- -3' miRNA: 3'- aCUGCa--------AGUGGGa---GGGUUGGCGUuag -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 126939 | 0.66 | 0.989778 |
Target: 5'- cUGGCGgu-GCCCUUCC-ACCGCu-UCu -3' miRNA: 3'- -ACUGCaagUGGGAGGGuUGGCGuuAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 124922 | 0.66 | 0.989778 |
Target: 5'- cGACGccaccUUCACgCUgCCCGACCcgGUGAUCg -3' miRNA: 3'- aCUGC-----AAGUGgGA-GGGUUGG--CGUUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 179966 | 0.66 | 0.988979 |
Target: 5'- aUGACGUucugggggucuuuguUCAUgCUCUCGcggGCCGCcGUCg -3' miRNA: 3'- -ACUGCA---------------AGUGgGAGGGU---UGGCGuUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 196372 | 0.66 | 0.98842 |
Target: 5'- gUGGCGUUgUACUCgcaucgCCgAGCCGCcGUCa -3' miRNA: 3'- -ACUGCAA-GUGGGa-----GGgUUGGCGuUAG- -5' |
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13951 | 3' | -53.3 | NC_003521.1 | + | 13199 | 0.66 | 0.98842 |
Target: 5'- aGAUGUUgcUCCUCCCGGCCcUggUCc -3' miRNA: 3'- aCUGCAAguGGGAGGGUUGGcGuuAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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