Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13963 | 3' | -61.3 | NC_003521.1 | + | 166271 | 0.66 | 0.803276 |
Target: 5'- -cGCCCUcagacauacuuguccACCacguaCGAGGGCUcCACCa- -3' miRNA: 3'- gaCGGGA---------------UGGg----GCUCCCGAuGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 18955 | 0.66 | 0.803276 |
Target: 5'- -cGCCCUACCCagcgacaacgaggaGGGGGUcgaggACGCgCCg -3' miRNA: 3'- gaCGGGAUGGGg-------------CUCCCGa----UGUG-GGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 112494 | 0.66 | 0.799912 |
Target: 5'- cCUGUaCCUG-CCCGAGgaccgcGGCUACACCa- -3' miRNA: 3'- -GACG-GGAUgGGGCUC------CCGAUGUGGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 208880 | 0.66 | 0.792264 |
Target: 5'- gUGCCUguuccuggACCCCGAGGaGCgcgaggucaucgcgcGCugCCUg -3' miRNA: 3'- gACGGGa-------UGGGGCUCC-CGa--------------UGugGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 16190 | 0.66 | 0.791408 |
Target: 5'- cCUGUCCgagugcugGCCCuuCGAGguGGCgGCGCCCUg -3' miRNA: 3'- -GACGGGa-------UGGG--GCUC--CCGaUGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 153489 | 0.66 | 0.791408 |
Target: 5'- aUGCCCUugCCgCGca-GCUGCGCCUc -3' miRNA: 3'- gACGGGAugGG-GCuccCGAUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 170603 | 0.66 | 0.788832 |
Target: 5'- -aGCCCUGCUgcgcgccaagcgcuUCGAGGGCcg-ACCCg -3' miRNA: 3'- gaCGGGAUGG--------------GGCUCCCGaugUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 127715 | 0.66 | 0.782777 |
Target: 5'- -cGCaCCUGgCCCGAGagccGCUccuGCACCCg -3' miRNA: 3'- gaCG-GGAUgGGGCUCc---CGA---UGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 204504 | 0.66 | 0.782777 |
Target: 5'- aUGUCCccacGCCCCGAcugaGGG-UGCGCCCc -3' miRNA: 3'- gACGGGa---UGGGGCU----CCCgAUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 66610 | 0.66 | 0.774026 |
Target: 5'- cCUGCCCgacccgcgcgACCCCaacguGGGCgcgucGCACCUg -3' miRNA: 3'- -GACGGGa---------UGGGGcu---CCCGa----UGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 170561 | 0.66 | 0.774026 |
Target: 5'- cCUGCgCCUG-CCCGu--GCUGCACCCc -3' miRNA: 3'- -GACG-GGAUgGGGCuccCGAUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 16803 | 0.66 | 0.774026 |
Target: 5'- -cGCCC-GCCCCGAccgccGGGCccgccGCugCCg -3' miRNA: 3'- gaCGGGaUGGGGCU-----CCCGa----UGugGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 5910 | 0.66 | 0.765162 |
Target: 5'- aCUGCUgUACCgCGAGGGgCUACGaugacgguUCCg -3' miRNA: 3'- -GACGGgAUGGgGCUCCC-GAUGU--------GGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 232554 | 0.66 | 0.765162 |
Target: 5'- gUGgCCUACUaCGGGGGCUGCugUg- -3' miRNA: 3'- gACgGGAUGGgGCUCCCGAUGugGga -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 55630 | 0.66 | 0.765162 |
Target: 5'- -gGCCC-ACaCCGAGGGCgucACGCuCCa -3' miRNA: 3'- gaCGGGaUGgGGCUCCCGa--UGUG-GGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 60734 | 0.66 | 0.765162 |
Target: 5'- gCUGCCCU-CCCUGgacGGGGUgACGCgCa -3' miRNA: 3'- -GACGGGAuGGGGC---UCCCGaUGUGgGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 205590 | 0.66 | 0.764269 |
Target: 5'- gUGCCgUcucgcGCCCCGAGGacGCUcgauccgacccgcGCGCCCc -3' miRNA: 3'- gACGGgA-----UGGGGCUCC--CGA-------------UGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 35969 | 0.66 | 0.756193 |
Target: 5'- gUGCCCgagaUCCGGGuGGaCUACACCUc -3' miRNA: 3'- gACGGGaug-GGGCUC-CC-GAUGUGGGa -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 138846 | 0.67 | 0.747127 |
Target: 5'- -gGCCUgcgGCCa-GAuGGGCUGCACCUUc -3' miRNA: 3'- gaCGGGa--UGGggCU-CCCGAUGUGGGA- -5' |
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13963 | 3' | -61.3 | NC_003521.1 | + | 112979 | 0.67 | 0.747127 |
Target: 5'- cCUGCCCgacgggcugGCgCCGGGGcccuuccacgaGCUGCGCgCCUg -3' miRNA: 3'- -GACGGGa--------UGgGGCUCC-----------CGAUGUG-GGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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