Results 1 - 20 of 51 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17003 | 5' | -58.5 | NC_004333.2 | + | 32828 | 0.66 | 0.546592 |
Target: 5'- aGGAACGccacgugcaacaugaGCG-CGGCCUGCuCGaGUCGGu -3' miRNA: 3'- gUCUUGC---------------CGCgGUUGGACG-GC-CAGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 9948 | 0.66 | 0.542389 |
Target: 5'- -cGAACGGCGCgGcgcGCUUGCuCGacUCGGg -3' miRNA: 3'- guCUUGCCGCGgU---UGGACG-GCc-AGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 44971 | 0.66 | 0.53193 |
Target: 5'- -cGGGCGGCGC--GCCggGCCGGcucacCGGc -3' miRNA: 3'- guCUUGCCGCGguUGGa-CGGCCa----GCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 39954 | 0.66 | 0.53193 |
Target: 5'- gCGGGcCGGCGUCAuCCUGCCGcGcagCGu -3' miRNA: 3'- -GUCUuGCCGCGGUuGGACGGC-Ca--GCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 17881 | 0.66 | 0.53193 |
Target: 5'- gCAGGcgcgACGGCgGCCAGCUcGCC-GUCGa -3' miRNA: 3'- -GUCU----UGCCG-CGGUUGGaCGGcCAGCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 17758 | 0.66 | 0.530889 |
Target: 5'- gCAGucgcuACGGCGCgCGAauucgguCCggcGCCGGUCGc -3' miRNA: 3'- -GUCu----UGCCGCG-GUU-------GGa--CGGCCAGCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 37106 | 0.66 | 0.521545 |
Target: 5'- cCGGAAUGG-GCCGcacGCCcGgCGGUCGa -3' miRNA: 3'- -GUCUUGCCgCGGU---UGGaCgGCCAGCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 9387 | 0.66 | 0.521545 |
Target: 5'- ---cGCGGCGCC--CCaUGCCGGacauuUCGGc -3' miRNA: 3'- gucuUGCCGCGGuuGG-ACGGCC-----AGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 10503 | 0.66 | 0.521545 |
Target: 5'- -----gGGCcgGCCGACgaGCgGGUCGGg -3' miRNA: 3'- gucuugCCG--CGGUUGgaCGgCCAGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 1642 | 0.66 | 0.521545 |
Target: 5'- --cGGCGGCGUagcccugaauGCgCUGCgCGGUCGGa -3' miRNA: 3'- gucUUGCCGCGgu--------UG-GACG-GCCAGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 23151 | 0.66 | 0.521545 |
Target: 5'- -cGAuguUGGUGCCAucGCCcgucacGUCGGUCGGu -3' miRNA: 3'- guCUu--GCCGCGGU--UGGa-----CGGCCAGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 29855 | 0.66 | 0.515352 |
Target: 5'- uGGAACGGCGUgAggaucgugcgcacgcGCgUGCCGG-CGa -3' miRNA: 3'- gUCUUGCCGCGgU---------------UGgACGGCCaGCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 27281 | 0.66 | 0.50102 |
Target: 5'- -uGAACGGuCGCC-ACgaGCCGG-CGa -3' miRNA: 3'- guCUUGCC-GCGGuUGgaCGGCCaGCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 30500 | 0.66 | 0.50102 |
Target: 5'- -cGAGCGuGCgGCCGACCaggcUGCCGGcCa- -3' miRNA: 3'- guCUUGC-CG-CGGUUGG----ACGGCCaGcc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 28759 | 0.67 | 0.490891 |
Target: 5'- -cGAGCGccGCGUCGACCgcGCgGGUCGcGg -3' miRNA: 3'- guCUUGC--CGCGGUUGGa-CGgCCAGC-C- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 25408 | 0.67 | 0.490891 |
Target: 5'- -cGAGC-GCGCCugcAGCgUGCCGG-CGGc -3' miRNA: 3'- guCUUGcCGCGG---UUGgACGGCCaGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 45568 | 0.67 | 0.480859 |
Target: 5'- uCGGugcucAUGGCGCuCGACCaGCCcGGUCGc -3' miRNA: 3'- -GUCu----UGCCGCG-GUUGGaCGG-CCAGCc -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 18176 | 0.67 | 0.480859 |
Target: 5'- -cGAGCGGCGCCGcauggugcucgcGCUcggcGCCGGU-GGc -3' miRNA: 3'- guCUUGCCGCGGU------------UGGa---CGGCCAgCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 4882 | 0.67 | 0.441779 |
Target: 5'- uCGGcACGaGCGCaUAGCCgGCCGGgaUCGGc -3' miRNA: 3'- -GUCuUGC-CGCG-GUUGGaCGGCC--AGCC- -5' |
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17003 | 5' | -58.5 | NC_004333.2 | + | 8648 | 0.67 | 0.441779 |
Target: 5'- uCGGcGCGGCGcCCGGCUUGCuCGugacggugccGUCGGc -3' miRNA: 3'- -GUCuUGCCGC-GGUUGGACG-GC----------CAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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