Results 1 - 20 of 95 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17008 | 5' | -58.9 | NC_004333.2 | + | 32255 | 0.66 | 0.484177 |
Target: 5'- uGGCaGCaGCGGCCgaaaGCCuuccggauGGCGCGg -3' miRNA: 3'- gCCGaCG-CGUCGGaaagUGG--------UCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 26387 | 0.66 | 0.484177 |
Target: 5'- uCGGUcGUGaUAGCgCUUggCugCAGCGCGg -3' miRNA: 3'- -GCCGaCGC-GUCG-GAAa-GugGUCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 45044 | 0.66 | 0.474072 |
Target: 5'- gCGGC-GCGCAaacGCUgaaCACC-GCGCGg -3' miRNA: 3'- -GCCGaCGCGU---CGGaaaGUGGuCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 31601 | 0.66 | 0.474072 |
Target: 5'- aCGaGUUGUccgGCAuGCCUUUCGaCAGCGUGa -3' miRNA: 3'- -GC-CGACG---CGU-CGGAAAGUgGUCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 5543 | 0.66 | 0.464074 |
Target: 5'- uGGCcgGCGaCGGCUUggCGCgAGUGCa -3' miRNA: 3'- gCCGa-CGC-GUCGGAaaGUGgUCGCGc -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 43635 | 0.66 | 0.464074 |
Target: 5'- aCGGCccgUGCGUccGGCCUgcacCACCAGgauCGCa -3' miRNA: 3'- -GCCG---ACGCG--UCGGAaa--GUGGUC---GCGc -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 20581 | 0.66 | 0.464074 |
Target: 5'- gGGUcGCGCcguGGUgUUUCugCAGgCGCGg -3' miRNA: 3'- gCCGaCGCG---UCGgAAAGugGUC-GCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 17747 | 0.66 | 0.464074 |
Target: 5'- aGGUggUGCGCaagcauucAGCCgg-CGCC-GCGCGg -3' miRNA: 3'- gCCG--ACGCG--------UCGGaaaGUGGuCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 5165 | 0.66 | 0.455171 |
Target: 5'- uCGaGCaGCGCAGCUUgcgccugauugaacaUCGCCugcaGGCGCGg -3' miRNA: 3'- -GC-CGaCGCGUCGGAa--------------AGUGG----UCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 14239 | 0.66 | 0.454187 |
Target: 5'- aCGGCgUGCGCGcgcGCCUcuUUCgaGCCgAGCgGCGg -3' miRNA: 3'- -GCCG-ACGCGU---CGGA--AAG--UGG-UCG-CGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 7848 | 0.66 | 0.454187 |
Target: 5'- gCGGcCUGCGcCGGCCaUUUCcaGCUcGCGCu -3' miRNA: 3'- -GCC-GACGC-GUCGG-AAAG--UGGuCGCGc -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 11344 | 0.66 | 0.454187 |
Target: 5'- aGGCgGCGCGGUUcgucgUCACCuugccGCGCu -3' miRNA: 3'- gCCGaCGCGUCGGaa---AGUGGu----CGCGc -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 24526 | 0.66 | 0.454187 |
Target: 5'- cCGGCgGCGUuccGCCUcaaggcgaggacUUCAaCGGCGCGu -3' miRNA: 3'- -GCCGaCGCGu--CGGA------------AAGUgGUCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 34218 | 0.66 | 0.454187 |
Target: 5'- cCGGCcGC-CGGCCUgaagUCGCCAagGCGg -3' miRNA: 3'- -GCCGaCGcGUCGGAa---AGUGGUcgCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 32295 | 0.66 | 0.444416 |
Target: 5'- gCGGCgaugucgacgUGCGuCAGCaggucggUCACgAGCGCGu -3' miRNA: 3'- -GCCG----------ACGC-GUCGgaa----AGUGgUCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 8780 | 0.66 | 0.444416 |
Target: 5'- uGGCgaaGuCGCcggcaauGCCUUgCGCCGGCGUGa -3' miRNA: 3'- gCCGa--C-GCGu------CGGAAaGUGGUCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 17386 | 0.66 | 0.434763 |
Target: 5'- uCGaGCaGCGCaucgAGCUgcUCGCgCAGCGCGg -3' miRNA: 3'- -GC-CGaCGCG----UCGGaaAGUG-GUCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 44974 | 0.66 | 0.434763 |
Target: 5'- gCGGC-GCGCcgGGCCggcUCACCGGCc-- -3' miRNA: 3'- -GCCGaCGCG--UCGGaa-AGUGGUCGcgc -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 8932 | 0.67 | 0.425234 |
Target: 5'- gCGGCUucguucgcgccgGCGCuGUCcaUCGCguGCGCGg -3' miRNA: 3'- -GCCGA------------CGCGuCGGaaAGUGguCGCGC- -5' |
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17008 | 5' | -58.9 | NC_004333.2 | + | 31075 | 0.67 | 0.415829 |
Target: 5'- uCGGCUGacagguagaccuUGUGGCCcggUUCGCCgauguuccGGCGCGg -3' miRNA: 3'- -GCCGAC------------GCGUCGGa--AAGUGG--------UCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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