Results 1 - 20 of 91 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18251 | 3' | -62.8 | NC_004681.1 | + | 44137 | 0.66 | 0.473601 |
Target: 5'- aGGccGCgGGCCugGCCCUCaC-CGGCCGCg -3' miRNA: 3'- gCCa-CG-CCGG--CGGGAG-GuGCUGGUGg -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 37675 | 0.66 | 0.473601 |
Target: 5'- ---aGCGGCCGCgcgucgcuUCUCCAauuCCGCCa -3' miRNA: 3'- gccaCGCCGGCG--------GGAGGUgcuGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 25350 | 0.66 | 0.464338 |
Target: 5'- ---aGCGcacccaaccGCCGCCgCgggcgCCAUGGCCGCCg -3' miRNA: 3'- gccaCGC---------CGGCGG-Ga----GGUGCUGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 40241 | 0.66 | 0.464338 |
Target: 5'- gCGGcgucGCGGCCuGCCUgUCCcggGCGGUCACCg -3' miRNA: 3'- -GCCa---CGCCGG-CGGG-AGG---UGCUGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 69105 | 0.66 | 0.464338 |
Target: 5'- uGGUGUGaagcGCCGCCUcgcCCGCGcCCugGCCu -3' miRNA: 3'- gCCACGC----CGGCGGGa--GGUGCuGG--UGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 51030 | 0.66 | 0.464338 |
Target: 5'- aGG-GCGucaUCGCCCUCgGCGACgGCg -3' miRNA: 3'- gCCaCGCc--GGCGGGAGgUGCUGgUGg -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 38214 | 0.66 | 0.464338 |
Target: 5'- uGGgGCGGCCGaagaCgUcCCGCGGCggCGCCa -3' miRNA: 3'- gCCaCGCCGGCg---GgA-GGUGCUG--GUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 45593 | 0.66 | 0.455171 |
Target: 5'- ---gGUGGCCGgugccUCCUCCGCGuCgGCCa -3' miRNA: 3'- gccaCGCCGGC-----GGGAGGUGCuGgUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 55979 | 0.66 | 0.446103 |
Target: 5'- cCGGUGCucGGCUgccagGCCCUCacgcagCGCGA-CGCCa -3' miRNA: 3'- -GCCACG--CCGG-----CGGGAG------GUGCUgGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 9751 | 0.66 | 0.446103 |
Target: 5'- aGGUGaccCGGCC-CCCUCacgaACGucaGCUACCu -3' miRNA: 3'- gCCAC---GCCGGcGGGAGg---UGC---UGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 35148 | 0.66 | 0.446103 |
Target: 5'- --cUGUGGuuGCCgUCCGCGGCgAgCa -3' miRNA: 3'- gccACGCCggCGGgAGGUGCUGgUgG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 38331 | 0.66 | 0.446103 |
Target: 5'- aCGGUGCcuauggcGCCGCCgCgggacgUCUuCGGCCGCCc -3' miRNA: 3'- -GCCACGc------CGGCGG-G------AGGuGCUGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 6010 | 0.66 | 0.440711 |
Target: 5'- aGGUcuGCGGCgucuacgacauCGCCCcucccaucguccacaUCCugGACCguGCCa -3' miRNA: 3'- gCCA--CGCCG-----------GCGGG---------------AGGugCUGG--UGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 47460 | 0.66 | 0.437137 |
Target: 5'- aCGaGUGCcaucaGGUCGCCgCgugCgACGGCCACUa -3' miRNA: 3'- -GC-CACG-----CCGGCGG-Ga--GgUGCUGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 3640 | 0.66 | 0.437137 |
Target: 5'- ---aGCaGGCCGCCCUCgCcuucgACGucGCCGCCc -3' miRNA: 3'- gccaCG-CCGGCGGGAG-G-----UGC--UGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 67347 | 0.66 | 0.437137 |
Target: 5'- gCGGUGCGGCggggccagcuUGUUCUUgACGACuuuCACCc -3' miRNA: 3'- -GCCACGCCG----------GCGGGAGgUGCUG---GUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 49455 | 0.66 | 0.437137 |
Target: 5'- aGGUGUGcGCCcaguggucagcGCCCgCgGCG-CCGCCg -3' miRNA: 3'- gCCACGC-CGG-----------CGGGaGgUGCuGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 49311 | 0.66 | 0.434467 |
Target: 5'- gCGG-GCGGucugcacaaccucaCCGUCCgCCACGGCggCGCCg -3' miRNA: 3'- -GCCaCGCC--------------GGCGGGaGGUGCUG--GUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 44582 | 0.66 | 0.428275 |
Target: 5'- ---cGCcGCCGCgCU-CGCGGCCGCCg -3' miRNA: 3'- gccaCGcCGGCGgGAgGUGCUGGUGG- -5' |
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18251 | 3' | -62.8 | NC_004681.1 | + | 5890 | 0.66 | 0.428275 |
Target: 5'- uGGgGCGGCCaGCguuCUUCCAC-AUCGCCg -3' miRNA: 3'- gCCaCGCCGG-CG---GGAGGUGcUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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