Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18258 | 5' | -58.6 | NC_004681.1 | + | 18192 | 0.66 | 0.65545 |
Target: 5'- gGUCcuGGAGCGccuccCG-GCGCACCCccGGGUg -3' miRNA: 3'- -CAGu-CCUUGCa----GCgCGCGUGGG--UCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 8921 | 0.66 | 0.65545 |
Target: 5'- cUCGGGuGCGUCGCGCagACCUucaucGGUg -3' miRNA: 3'- cAGUCCuUGCAGCGCGcgUGGGu----CCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 40245 | 0.66 | 0.65545 |
Target: 5'- aUCuGcGGCGUCGCGgccUGCcuguCCCGGGCg -3' miRNA: 3'- cAGuCcUUGCAGCGC---GCGu---GGGUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 61562 | 0.66 | 0.644945 |
Target: 5'- gGUCAgaacGGAACGUCGUcauCGC-CCCAcuuguuGGCg -3' miRNA: 3'- -CAGU----CCUUGCAGCGc--GCGuGGGU------CCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 57207 | 0.66 | 0.634428 |
Target: 5'- --aGGGGGCGgaUCGC-CGC-CCCAGGa -3' miRNA: 3'- cagUCCUUGC--AGCGcGCGuGGGUCCg -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 61281 | 0.66 | 0.622859 |
Target: 5'- --gAGGAAUGUgGCGuCGCcgucgacccaguuGCCCuGGGCg -3' miRNA: 3'- cagUCCUUGCAgCGC-GCG-------------UGGG-UCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 66191 | 0.66 | 0.613399 |
Target: 5'- -gCAGGccguccuCGUUGCGCGCAUaCAGcGCg -3' miRNA: 3'- caGUCCuu-----GCAGCGCGCGUGgGUC-CG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 66011 | 0.66 | 0.613399 |
Target: 5'- cGUgAGGGACcUUGCG-GuCGCCCAGGa -3' miRNA: 3'- -CAgUCCUUGcAGCGCgC-GUGGGUCCg -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 18383 | 0.66 | 0.602902 |
Target: 5'- aGUCgAGGAcuGCGgcCGUGcCGCGCCCGGaGUu -3' miRNA: 3'- -CAG-UCCU--UGCa-GCGC-GCGUGGGUC-CG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 64251 | 0.67 | 0.592429 |
Target: 5'- -cCAuGGAaaGCGUUGUGCGC-CaCAGGCa -3' miRNA: 3'- caGU-CCU--UGCAGCGCGCGuGgGUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 47485 | 0.67 | 0.592429 |
Target: 5'- -gCAGGAccGCGUCGCcgugGCGCGuuggCGGGCg -3' miRNA: 3'- caGUCCU--UGCAGCG----CGCGUgg--GUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 7401 | 0.67 | 0.592429 |
Target: 5'- ---uGGAACGUCGCugGCGUGCagcucgucgCAGGCg -3' miRNA: 3'- caguCCUUGCAGCG--CGCGUGg--------GUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 70094 | 0.67 | 0.592429 |
Target: 5'- aUCAGaGAC--CGCGCGCACaCGGGCc -3' miRNA: 3'- cAGUCcUUGcaGCGCGCGUGgGUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 43364 | 0.67 | 0.581986 |
Target: 5'- -cCGGGGACGUCauauccccgGCGCGCAcaguCCCugAGaGCa -3' miRNA: 3'- caGUCCUUGCAG---------CGCGCGU----GGG--UC-CG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 52992 | 0.67 | 0.581986 |
Target: 5'- -gCAGGAuuuggauuUGUCGC-CGCGgCUCGGGCa -3' miRNA: 3'- caGUCCUu-------GCAGCGcGCGU-GGGUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 24685 | 0.67 | 0.581986 |
Target: 5'- gGUCgaaGGGAAUcuUCGagaugaugcCGCGCACCCAGuGCg -3' miRNA: 3'- -CAG---UCCUUGc-AGC---------GCGCGUGGGUC-CG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 13556 | 0.67 | 0.580943 |
Target: 5'- aUCAuGGGcgaagagACGUaCGCGCGCuucaagGCCgCGGGCg -3' miRNA: 3'- cAGU-CCU-------UGCA-GCGCGCG------UGG-GUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 46874 | 0.67 | 0.57158 |
Target: 5'- -gCGGGAugaccgugACGcCGUGCGCGCgCAGcGCc -3' miRNA: 3'- caGUCCU--------UGCaGCGCGCGUGgGUC-CG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 38786 | 0.67 | 0.565359 |
Target: 5'- cUCAuGGAacuGCGggcCGCGCGCAaguccgccgagaccaUCCGGGCc -3' miRNA: 3'- cAGU-CCU---UGCa--GCGCGCGU---------------GGGUCCG- -5' |
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18258 | 5' | -58.6 | NC_004681.1 | + | 61145 | 0.67 | 0.561221 |
Target: 5'- uGUCcaacGAACGcCGcCGCGaCGCCCAGGg -3' miRNA: 3'- -CAGuc--CUUGCaGC-GCGC-GUGGGUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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