Results 1 - 20 of 72 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18757 | 3' | -56.5 | NC_004683.1 | + | 21530 | 0.66 | 0.69948 |
Target: 5'- uCCCGGUCuGGuGCCGCuacaucgacgugAUCCuGCugGGcgGCg -3' miRNA: 3'- -GGGUUAG-CC-CGGCG------------UAGG-CGugCU--UG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 51177 | 0.66 | 0.695221 |
Target: 5'- aUCCGG-CGcGCCGCcgagaacgcgccCCGCACGAGCg -3' miRNA: 3'- -GGGUUaGCcCGGCGua----------GGCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 40584 | 0.66 | 0.68239 |
Target: 5'- uCUCGAUgGcGGCUGUccuggcacuggcgguGUUCGCAUGAGCa -3' miRNA: 3'- -GGGUUAgC-CCGGCG---------------UAGGCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 12691 | 0.66 | 0.674874 |
Target: 5'- aUCCGAggCGGGCacgGCAUCCucggcauccucggcGCGCGGGg -3' miRNA: 3'- -GGGUUa-GCCCGg--CGUAGG--------------CGUGCUUg -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 6562 | 0.66 | 0.667339 |
Target: 5'- aCCCGGcUCGGcGCUgGCGUCCucgacgcugGCGCGAcgACg -3' miRNA: 3'- -GGGUU-AGCC-CGG-CGUAGG---------CGUGCU--UG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 26461 | 0.66 | 0.667339 |
Target: 5'- aCCgCGAUCcaGGCCGC--CCGC-CGAGCc -3' miRNA: 3'- -GG-GUUAGc-CCGGCGuaGGCGuGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 2278 | 0.66 | 0.667339 |
Target: 5'- uCUCGAcgcgaGGcGCUGCAccCCGCAUGAGCa -3' miRNA: 3'- -GGGUUag---CC-CGGCGUa-GGCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 49915 | 0.66 | 0.656547 |
Target: 5'- aUCGAUCGGaCCGCAUgC-CACGAGg -3' miRNA: 3'- gGGUUAGCCcGGCGUAgGcGUGCUUg -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 11498 | 0.66 | 0.656547 |
Target: 5'- gCUGAagGuGGCCcCA-CCGCACGAGCa -3' miRNA: 3'- gGGUUagC-CCGGcGUaGGCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 36016 | 0.66 | 0.645734 |
Target: 5'- -aCGAUCGcGGCCcCuuGUCCGCuuGAACa -3' miRNA: 3'- ggGUUAGC-CCGGcG--UAGGCGugCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 35205 | 0.66 | 0.645734 |
Target: 5'- aUCCAAUCGGGacaagcaCGCAUCCauguCGGAa -3' miRNA: 3'- -GGGUUAGCCCg------GCGUAGGcgu-GCUUg -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 17323 | 0.66 | 0.645734 |
Target: 5'- cCCCGuaCGGcCCGUAUgCGUugGGGCg -3' miRNA: 3'- -GGGUuaGCCcGGCGUAgGCGugCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 42590 | 0.66 | 0.645734 |
Target: 5'- -gCGGUCgGGGCCGguCGUCCGgCugGggUg -3' miRNA: 3'- ggGUUAG-CCCGGC--GUAGGC-GugCuuG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 17861 | 0.66 | 0.645734 |
Target: 5'- aCCCAAccgggCGGGCCGgGgacgCCGCA-GcGCg -3' miRNA: 3'- -GGGUUa----GCCCGGCgUa---GGCGUgCuUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 32796 | 0.66 | 0.645734 |
Target: 5'- cUCCGAagucgCGGGCCaGC--CgGCGCGAGCu -3' miRNA: 3'- -GGGUUa----GCCCGG-CGuaGgCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 22615 | 0.66 | 0.645734 |
Target: 5'- aCCAGuugauUCGGGgCGCAUgugCCaGCACGAc- -3' miRNA: 3'- gGGUU-----AGCCCgGCGUA---GG-CGUGCUug -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 20298 | 0.66 | 0.641405 |
Target: 5'- aCCCGGcCgGGGCggcgacggcggcagCGCGUucaugucCCGCGCGAACa -3' miRNA: 3'- -GGGUUaG-CCCG--------------GCGUA-------GGCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 44933 | 0.67 | 0.634909 |
Target: 5'- cUCCAGgcCGGGCCaccacgGCGaUUGCGCGAGCu -3' miRNA: 3'- -GGGUUa-GCCCGG------CGUaGGCGUGCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 24417 | 0.67 | 0.634909 |
Target: 5'- aCgAGUCGGcGuuGCAUCuCGCGggUGAACu -3' miRNA: 3'- gGgUUAGCC-CggCGUAG-GCGU--GCUUG- -5' |
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18757 | 3' | -56.5 | NC_004683.1 | + | 10876 | 0.67 | 0.634909 |
Target: 5'- uCCCGcugAUCcGaGCCGCAgaaGCGCGGACg -3' miRNA: 3'- -GGGU---UAGcC-CGGCGUaggCGUGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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