Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19274 | 3' | -61.6 | NC_004685.1 | + | 48085 | 0.66 | 0.483596 |
Target: 5'- uGGC-ACCGAacgugcCGACCCaGCCguCGCCCGa -3' miRNA: 3'- -UUGaUGGCUga----GCUGGG-CGG--GCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 20070 | 0.66 | 0.483596 |
Target: 5'- cGACcACCuGGCguucgUCGGCCaGCCCGCCUa -3' miRNA: 3'- -UUGaUGG-CUG-----AGCUGGgCGGGCGGGc -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 11225 | 0.66 | 0.482635 |
Target: 5'- uACggACCGuucacugaggaucACUCGuguCCUGCCUGCCUGg -3' miRNA: 3'- uUGa-UGGC-------------UGAGCu--GGGCGGGCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 64226 | 0.66 | 0.474027 |
Target: 5'- ---cGCCGGa-UGACCCGCgCCGgCCCGc -3' miRNA: 3'- uugaUGGCUgaGCUGGGCG-GGC-GGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 2226 | 0.66 | 0.464555 |
Target: 5'- uGGgUGCCGGgUgccaGACCUGCcugCCGCCCGg -3' miRNA: 3'- -UUgAUGGCUgAg---CUGGGCG---GGCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 8511 | 0.66 | 0.464555 |
Target: 5'- cGCUACCGgaugggccgcAgUCGACCaGCCgGCUCGa -3' miRNA: 3'- uUGAUGGC----------UgAGCUGGgCGGgCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 36850 | 0.66 | 0.464555 |
Target: 5'- uGAUcGCgCGGCUCGACaCCGCggCGUCCGg -3' miRNA: 3'- -UUGaUG-GCUGAGCUG-GGCGg-GCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 48222 | 0.66 | 0.464555 |
Target: 5'- cGACgcCCGugUCGAacaCCGgCCGCCa- -3' miRNA: 3'- -UUGauGGCugAGCUg--GGCgGGCGGgc -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 55659 | 0.66 | 0.463614 |
Target: 5'- cGGCUGCCGuuggcgaACUCGA-CCGCCuCGUcgCCGg -3' miRNA: 3'- -UUGAUGGC-------UGAGCUgGGCGG-GCG--GGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 48329 | 0.66 | 0.455183 |
Target: 5'- cAGCUGCCG--UCGGCCUGaauCgGCCCGu -3' miRNA: 3'- -UUGAUGGCugAGCUGGGCg--GgCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 27914 | 0.66 | 0.455183 |
Target: 5'- uGCUGCgucGCUCGACCgCGCCgucaaCGCCCc -3' miRNA: 3'- uUGAUGgc-UGAGCUGG-GCGG-----GCGGGc -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 32532 | 0.66 | 0.455183 |
Target: 5'- gAACcuucCCGGCgUCGGCCCgGCCaCGCCgGa -3' miRNA: 3'- -UUGau--GGCUG-AGCUGGG-CGG-GCGGgC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 43821 | 0.66 | 0.445913 |
Target: 5'- ----cCCGACcugcgCGGgCCGCUCGCCCa -3' miRNA: 3'- uugauGGCUGa----GCUgGGCGGGCGGGc -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 11336 | 0.66 | 0.445913 |
Target: 5'- --gUGCgCGACcggCGACCCGCcCCGacaCCGa -3' miRNA: 3'- uugAUG-GCUGa--GCUGGGCG-GGCg--GGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 68586 | 0.66 | 0.445913 |
Target: 5'- aGGCUGaCGACUgaUGcCCCGCauCCGCCCGc -3' miRNA: 3'- -UUGAUgGCUGA--GCuGGGCG--GGCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 7023 | 0.66 | 0.43675 |
Target: 5'- gGAgaAUCGGCUCGGUCUGCUCGCCgCGc -3' miRNA: 3'- -UUgaUGGCUGAGCUGGGCGGGCGG-GC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 49561 | 0.67 | 0.431304 |
Target: 5'- cAGCaACCGAucCUCGccgcgcaucgccagcACCCGCgCGCCCu -3' miRNA: 3'- -UUGaUGGCU--GAGC---------------UGGGCGgGCGGGc -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 36380 | 0.67 | 0.427696 |
Target: 5'- cGCgcCCGACggCGGCCUGggcaacuucCCCGCCCu -3' miRNA: 3'- uUGauGGCUGa-GCUGGGC---------GGGCGGGc -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 10238 | 0.67 | 0.427696 |
Target: 5'- gAGCUgACCGucaUCGACUgGCCgcUGCCCGa -3' miRNA: 3'- -UUGA-UGGCug-AGCUGGgCGG--GCGGGC- -5' |
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19274 | 3' | -61.6 | NC_004685.1 | + | 36458 | 0.67 | 0.418754 |
Target: 5'- ---cAUCGACUUcacCCCGCUCGCCUGg -3' miRNA: 3'- uugaUGGCUGAGcu-GGGCGGGCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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