Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19561 | 3' | -62.5 | NC_004686.1 | + | 3823 | 0.66 | 0.338499 |
Target: 5'- gUCGUAccAGUC-CGcCGCCGCCGCCAu -3' miRNA: 3'- aGGCGU--UCGGuGCcGUGGCGGCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 47329 | 0.66 | 0.338499 |
Target: 5'- uUCCGCA-GCCGaaccGCGCCaCCGUCGAg -3' miRNA: 3'- -AGGCGUuCGGUgc--CGUGGcGGCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 43727 | 0.66 | 0.330657 |
Target: 5'- -aCGUuGGUCGCGGCacgcaaccuggGCCGCCGCg-- -3' miRNA: 3'- agGCGuUCGGUGCCG-----------UGGCGGCGguu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 42872 | 0.66 | 0.32833 |
Target: 5'- gUCCGCcuGCCugGcuuccaacuccugaGCcCgCGCCGCCAAc -3' miRNA: 3'- -AGGCGuuCGGugC--------------CGuG-GCGGCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 9773 | 0.66 | 0.322949 |
Target: 5'- uUCUGCu-GCCAa--CACUGCCGCCAc -3' miRNA: 3'- -AGGCGuuCGGUgccGUGGCGGCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 22736 | 0.66 | 0.322949 |
Target: 5'- cCCGCcuaacCCcCGGCGCCGCgGCUAu -3' miRNA: 3'- aGGCGuuc--GGuGCCGUGGCGgCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 56151 | 0.66 | 0.322949 |
Target: 5'- gUCGCGGGuCCACcggucgcgGGUGCCGaaCCGCCAGg -3' miRNA: 3'- aGGCGUUC-GGUG--------CCGUGGC--GGCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 12422 | 0.66 | 0.322949 |
Target: 5'- gUCGUcguGGCCGcCGGCAuaGCCGCCc- -3' miRNA: 3'- aGGCGu--UCGGU-GCCGUggCGGCGGuu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 28881 | 0.66 | 0.322186 |
Target: 5'- aUCCaGCAGGaacaccaacaacaCCGCGGCGacacCCGCCGCa-- -3' miRNA: 3'- -AGG-CGUUC-------------GGUGCCGU----GGCGGCGguu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 51737 | 0.66 | 0.319904 |
Target: 5'- uUCCGCu-GCgGCGGCACCGaccucuucagggaCGUCGg -3' miRNA: 3'- -AGGCGuuCGgUGCCGUGGCg------------GCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 10133 | 0.66 | 0.314626 |
Target: 5'- aCCGCGcgcuggaAGuCCGCGGCAgaaaccUUGCCGUCAGa -3' miRNA: 3'- aGGCGU-------UC-GGUGCCGU------GGCGGCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 12332 | 0.66 | 0.307938 |
Target: 5'- gCCGCAAGggGCGGCuauGCCGgCgGCCAc -3' miRNA: 3'- aGGCGUUCggUGCCG---UGGC-GgCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 22471 | 0.66 | 0.307938 |
Target: 5'- -aUGCGguuguAGCCAuCGGUGCCgguGCCGCCAu -3' miRNA: 3'- agGCGU-----UCGGU-GCCGUGG---CGGCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 39450 | 0.67 | 0.300635 |
Target: 5'- --gGCAGGCCACGaucucggcGCAuuuCCGCgGCCAGg -3' miRNA: 3'- aggCGUUCGGUGC--------CGU---GGCGgCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 54845 | 0.67 | 0.293466 |
Target: 5'- uUCCGUGA-CCGCGGUaaggacccgcGCCGgcCCGCCAAc -3' miRNA: 3'- -AGGCGUUcGGUGCCG----------UGGC--GGCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 49962 | 0.67 | 0.293466 |
Target: 5'- aUCGUuucGCCGCGGCcuUCGUCGCCGg -3' miRNA: 3'- aGGCGuu-CGGUGCCGu-GGCGGCGGUu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 14584 | 0.67 | 0.293466 |
Target: 5'- gCCGC--GCCACGGCuCCGgaUCGCCu- -3' miRNA: 3'- aGGCGuuCGGUGCCGuGGC--GGCGGuu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 5620 | 0.67 | 0.286431 |
Target: 5'- cUCCGgGccGCCACGGacgguguccgaCACCGCCGCg-- -3' miRNA: 3'- -AGGCgUu-CGGUGCC-----------GUGGCGGCGguu -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 14510 | 0.67 | 0.27953 |
Target: 5'- aUCCG-GAGCCGUGGCGCgGC-GCCAAc -3' miRNA: 3'- -AGGCgUUCGGUGCCGUGgCGgCGGUU- -5' |
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19561 | 3' | -62.5 | NC_004686.1 | + | 34732 | 0.67 | 0.277486 |
Target: 5'- gUUCGCcGGCUACGGCaACCGCaaggaacgucucuaCGCCu- -3' miRNA: 3'- -AGGCGuUCGGUGCCG-UGGCG--------------GCGGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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